Non-bleaching fluorescence emission difference microscopy using single 808-nm laser excited red upconversion emission
Non-bleaching fluorescence emission difference microscopy using single 808-nm laser excited red upconversion emission
复制标题
使用单 808 nm 激光激发红色上转换发射的非漂白荧光发射差异显微镜
DOI：
10.1364/oe.25.030885
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发表时间：
2017-12-11
期刊：
影响因子：
3.8
通讯作者：
Zhan, Qiuqiang
中科院分区：
文献类型：
--
作者：
Wu, Qiusheng;Huang, Bingru;Zhan, Qiuqiang
光学超分辨率显微镜已经成为一种强大的技术，帮助科学家在纳米尺度上监测感兴趣的样品。荧光发射差分（FED）显微术是一种非常简单的超分辨方法，它不需要很高的激光强度，并且与介质种类无关，这使得FED显微术具有很大的潜力。然而，迄今为止，FED显微镜中应用的生物标志物通常遭受光漂白问题。在这项工作中，通过引入Er 3+激活的上转换纳米粒子的红色发射和非光漂白性能，我们证明了非漂白超分辨率成像与FED显微镜。掺杂剂钕离子（Nd 3+）可以作为高效的敏化离子，并能够以相对较低的功率激发近红外808 nm CW激光，这可能会减少高强度/短波长光引起的组织损伤。对单分散NaYF 4：Nd 3 +/Yb 3 +/Er 3 +@NaYF4：Nd 3 + UCNPs的模拟和实验也表明，这些UCNPs的多光子特性的容易饱和有利于FED显微镜的分辨率提高。(C)根据OSA开放获取出版协议的条款，2017年美国光学学会
Optical super-resolution microscopy has become a powerful technique to help scientists to monitor the sample of interest at nanoscale. Fluorescence emission difference (FED) microscopy, a very facile super-resolution method, does not require high depleting laser intensity and is independent on the species of agents, which makes FED microscopy possess great potential. However, to date, the biomarkers applied in FED microscopy usually suffer from a photo-bleaching problem. In this work, by introducing Er3+ activated upconverting nanoparticles with red-color emission and non-photobleaching properties, we demonstrate nonbleaching super-resolution imaging with FED microscopy. The dopant neodymium ions (Nd3+) can work as highly efficient sensitizing ions and enable near infrared 808-nm CW laser excitation of relatively low power, which would potentially reduce high intensity/short-wavelength light induced tissue damage. Both simulations and experiments on monodispersed NaYF4:Nd3+/Yb3+/Er3+@NaYF4:Nd3+ UCNPs also indicate that the easy saturation of the multiphoton properties of these UCNPs is beneficial to resolution enhancement in FED microscopy. (C) 2017 Optical Society of America under the terms of the OSA Open Access Publishing Agreement