Noncontact 3-D Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution.
Noncontact 3-D Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution.
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DOI：
10.1109/tmi.2017.2708661
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发表时间：
2017-10
影响因子：
10.6
通讯作者：
Yu G
中科院分区：
文献类型：
--
作者：
Huang C;Irwin D;Zhao M;Shang Y;Agochukwu N;Wong L;Yu G
近红外漫射相关技术和仪器的最新进展为多功能深层组织微血管血流成像系统开辟了道路。尽管取得了这些进展，但仍然需要一种完全非接触式，非侵入性的设备，具有从小型/测试（动物）到大型/目标（人类）的高可翻译性，并且在两者上都有微不足道的应用。因此，我们讨论了我们新开发的满足这一需求的装置，称为非接触散斑对比弥散相关断层扫描（nc_scDCT）。nc_scDCT提供快速、连续、便携、无创和廉价的三维断层扫描深层（高达10毫米）组织血流分布，具有简单的设计和定制。所提出的特点包括结合复杂组织边界的有限元方法实现，避免组织压缩和相互作用的完全非接触式硬件，使用漫射散斑对比方法的快速数据收集，基于反射的设计促进实验翻译，相关技术的可扩展性，稳健的可调源和检测器模式和密度，用于高分辨率测量，具有灵活的感兴趣区域，可实现独特的特定应用设置。验证显示在使用带有泵连接管（高）和幻影球体（低）的组织幻影相对于背景的高对比度和低对比度的检测和表征中。此外，在体内验证提取时空三维血流分布和充血反应在前臂袖带闭塞。最后，通过乳房皮瓣切除术术中影像学检查器械在临床应用的可行性。
Recent advancements in near-infrared diffuse correlation techniques and instrumentation have opened the path for versatile deep tissue microvasculature blood flow imaging systems. Despite this progress there remains a need for a completely noncontact, noninvasive device with high translatability from small/testing (animal) to large/target (human) subjects with trivial application on both. Accordingly, we discuss our newly developed setup which meets this demand, termed noncontact speckle contrast diffuse correlation tomography (nc_scDCT). The nc_scDCT provides fast, continuous, portable, noninvasive, and inexpensive acquisition of three-dimensional tomographic deep (up to 10 mm) tissue blood flow distributions with straightforward design and customization. The features presented include a finite-element-method implementation for incorporating complex tissue boundaries, fully noncontact hardware for avoiding tissue compression and interactions, rapid data collection with a diffuse speckle contrast method, reflectance-based design promoting experimental translation, extensibility to related techniques, and robust adjustable source and detector patterns and density for high resolution measurement with flexible regions of interest enabling unique application-specific setups. Validation is shown in the detection and characterization of both high and low contrasts in flow relative to the background using tissue phantoms with a pump-connected tube (high) and phantom spheres (low). Furthermore, in vivo validation of extracting spatiotemporal three-dimensional blood flow distributions and hyperemic response during forearm cuff occlusion is demonstrated. Finally, the success of instrument feasibility in clinical use is examined through intraoperative imaging of mastectomy skin flap.