A simple reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of curcumin in aqueous humor of rabbit
A simple reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of curcumin in aqueous humor of rabbit
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DOI：
10.4103/2231-4040.137448
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发表时间：
2014-07-01
影响因子：
1.4
通讯作者：
Mandal, Tapan Kumar
中科院分区：
文献类型：
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作者：
Mishra, Akhilesh;Dewangan, Gayatri;Mandal, Tapan Kumar
本文描述了一种使用高效液相色谱法（HPLC）测定兔房水中姜黄素（二阿魏酰甲烷）的简便快速方法。采用C - 18柱（250×4.6 mm，5μm粒径），以含25 mM磷酸二氢钾（pH 3.5）：乙腈（40∶60）的流动相进行等度洗脱，并使用光电二极管阵列（PDA）检测器在424 nm处检测姜黄素。姜黄素的回归数据在0.1 - 10μg/ml浓度范围内呈现良好的线性关系，r²>0.998。该测定方法的日内和日间变异系数的相对标准偏差（RSD）分别小于5.0和8.5。方法的回收率在79.8% - 83.6%之间。姜黄素的定量限为0.01μg/ml。该方法具有良好的准确性、精密度和定量限。还得出结论，该方法可用于测量房水中极低浓度的姜黄素。
This article describes a simple and rapid method for determination of curcumin (diferuloylmethane) in aqueous humor of rabbit using high-performance liquid chromatography (HPLC). Analysis was performed using a C- 18 column (250 x 4.6 mm, 5 mu luna) by isocratic elution with a mobile phase containing 25 mM potassium dihydrogen orthophosphate (pH 3.5): Acetonitrile (40: 60) and detection at 424 nm using a photodiode array (PDA) detector for curcumin. The regression data for curcumin showed a good linear relationship with r(2) > 0.998 over the concentration range of 0.1-10 mu g ml(-1). Relative standard deviations (RSD) for the intraday and interday coefficient of variations for the assay were less than 5.0 and 8.5, respectively. The recovery of the method was between 79.8-83.6%. The quantification limit of the method for curcumin was 0.01 mu g ml(-1). This method has good accuracy, precision, and quantitation limit. It is also concluded that the method is useful for measuring very low curcumin concentrations in aqueous humor.