Barley stripe mosaic virus γb subverts autophagy to promote viral infection by disrupting the ATG7-ATG8 interaction
Barley stripe mosaic virus γb subverts autophagy to promote viral infection by disrupting the ATG7-ATG8 interaction
复制标题
大麦条纹花叶病毒γb通过破坏ATG7-ATG8相互作用来颠覆自噬以促进病毒感染
DOI：
10.1105/tpc.18.00122
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发表时间：
2018
期刊：
影响因子：
11.6
通讯作者：
Li Dawei
中科院分区：
文献类型：
--
作者：
Yang Meng;Zhang Yongliang;Xie Xialin;Yue Ning;Li Jinlin;Wang Xian Bing;Han Chenggui;Yu Jialin;Liu Yule;Li Dawei
Autophagy is a conserved defense strategy against viral infection. However, little is known about the counterdefense strategies of plant viruses involving interference with autophagy. Here, we show that γb protein fromBarley stripe mosaic virus(BSMV), a positive single-stranded RNA virus, directly interacts with AUTOPHAGY PROTEIN7 (ATG7). BSMV infection suppresses autophagy, and overexpression of γb protein is sufficient to inhibit autophagy. Furthermore, silencing of autophagy-related geneATG5andATG7inNicotiana benthamianaplants enhanced BSMV accumulation and viral symptoms, indicating that autophagy plays an antiviral role in BSMV infection. Molecular analyses indicated that γb interferes with the interaction of ATG7 with ATG8 in a competitive manner, whereas a single point mutation in γb, Tyr29Ala (Y29A), made this protein deficient in the interaction with ATG7, which was correlated with the abolishment of autophagy inhibition. Consistently, the mutant BSMVY29Avirus showed reduced symptom severity and viral accumulation. Taken together, our findings reveal that BSMV γb protein subverts autophagy-mediated antiviral defense by disrupting the ATG7-ATG8 interaction to promote plant RNA virus infection, and they provide evidence that ATG7 is a target of pathogen effectors that functions in the ongoing arms race of plant defense and viral counterdefense.
