Roles of adherence and matrix metalloproteinases in growth patterns of fungal pathogens in cornea (Retracted Article)
Roles of adherence and matrix metalloproteinases in growth patterns of fungal pathogens in cornea (Retracted Article)
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DOI：
10.1080/02713680590968196
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发表时间：
2005-08-01
影响因子：
2
通讯作者：
Xie, LX
中科院分区：
文献类型：
--
作者：
Dong, XG;Shi, WY;Xie, LX
目的：探讨粘附性和基质金属蛋白酶（MMPs）在角膜主要真菌致病菌生长模式中的作用。研究方法：将96只家兔的96只眼分为4组，分别接种烟曲霉、白色念珠菌、茄病镰刀菌和桔绿青霉的分生孢子，诱发真菌性角膜炎。在接种后2，8，16小时，1，2，3，5和8天，每组角膜进行扫描电镜，组织病理学检查，明胶酶谱。另取8只兔的8只眼作为对照。结果：真菌接种组均发生真菌性角膜炎。分生孢子与角膜上皮基底膜的结合较早。和C. albicans组比F. solani和P. citreo-viride类群。1 ~ 3天开始基底膜破坏。组织学上，A组炎性细胞浸润较B组明显。和C. albicans组比F. solani和P. citreo-viride组。A.和C.白色念珠菌的菌丝在垂直于角膜基质层的平面内穿过角膜，而F. solani和P. citreo-viride平行于角膜层。MMP-9和MMP-2在所有感染的角膜中均有表达。在第3天，蛋白水解最活跃，MMP-9的水平在A.和C. albicans组比F. solani和P. citreo-viride类群。分生孢子粘附数与炎症程度、MMP-9水平呈正相关（p < 0.05）。结论.不同真菌感染眼的粘附能力、对中性粒细胞的趋化性和MMP-9表达水平不同，这可能是导致真菌在角膜中不同生长模式的原因。
Purpose: To investigate the roles of adherence and matrix metalloproteinases (MMPs) in growth patterns of major fungal pathogens in cornea. Methods: Ninety-six eyes in 96 rabbits were equally divided into four groups receiving inoculation of fungal conidia of Aspergillus fumigatus, Candida albicans, Fusarium solani, and Penicillium citreo-viride, respectively, to induce fungal keratitis. Corneas in each group were obtained at 2, 8, 16 hr, and 1, 2, 3, 5, and 8 days after inoculation and were subjected to scanning electron microscopy, histopathological examination, and gelatin zymography. Eight saline-inoculated eyes in another eight rabbits served as controls. Results: All eyes in the fungus-inoculated groups developed fungal keratitis. The binding of conidia to corneal epithelial basement membrane was initiated earlier in the A. fumigatus and C. albicans groups than in the F. solani and P. citreo-viride groups. Destruction of basement membrane began at 1 to 3 days. Histopathologically, infiltration of inflammatory cells was more evident in the A. fumigatus and C. albicans groups than the F. solani and P. citreo-viride groups at 3 days. The hyphae of A. fumigatus and C. albicans traversed the cornea in a plane perpendicular to the stromal lamellae, whereas the hyphae of F. solani and P. citreo-viride lay parallel to the corneal lamellae. MMP-9 and MMP-2 were found in all infected corneas. At 3 days, proteolysis was most active; the level of MMP-9 was higher in the A. fumigatus and C. albicans groups than in the F. solani and P. citreo-viride groups. There were positive correlations among the number of binding conidia, degree of inflammation, and level of MMP-9 (p < 0.05). Conclusions. The adherence ability, chemotaxis to neutrophils, and MMP-9 expression level differ in eyes with different fungal pathogens, which may contribute to the different growth patterns of fungi in cornea.