Subspecies- and species-specific antigens of Leishmania mexicana characterized by monoclonal antibodies.
Subspecies- and species-specific antigens of Leishmania mexicana characterized by monoclonal antibodies.
复制标题
以单克隆抗体为特征的墨西哥利什曼原虫亚种和种特异性抗原。
作者：
D. Pratt;E. Bennett;G. Grimaldi;C. Jaffe
针对墨西哥利什曼原虫(L.miciana miciana，L11，M379)、墨西哥利什曼原虫(L.miciana amazonensis，WR303，H6，Lv72)和墨西哥利什曼原虫参考种群(L.miciana pifanoi，L20)的特异性单抗已经研制成功。通过使用间接放射免疫结合试验和107份新世界利什曼原虫的分析，证实了这些抗体的特异性。与这些种和亚种特异的决定因素相关的分子已经通过蛋白质印迹分析进行了表征，并且主要由低分子量组成。(11,000至50,000)膜相关成分。
Monoclonal antibodies have been produced that are specific for the reference stocks of Leishmania mexicana species and subspecies L. mexicana mexicana(L11, M379), L. mexicana amazonensis (WR303, H6, LV72), and L. mexicana pifanoi (L20). The specificities of these antibodies were confirmed by analyses employing an indirect radioimmune binding assay and 107 stocks of New World Leishmania. The molecules associated with these species- and subspecies-specific determinants have been characterized by Western blot analysis and consist of mainly low m.w. (11,000 to 50,000) membrane-associated components.