EVIDENCE FOR A RENAL VASCULAR RECEPTOR IN CONTROL OF RENIN SECRETION
EVIDENCE FOR A RENAL VASCULAR RECEPTOR IN CONTROL OF RENIN SECRETION
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DOI：
10.1152/ajplegacy.1971.220.6.1593
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发表时间：
1971-01-01
影响因子：
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通讯作者：
PREWITT, RL
中科院分区：
文献类型：
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作者：
BLAINE, EH;DAVIS, JO;PREWITT, RL
材料与方法设计犬失神经、无抖动肾脏失血后血浆肾素活性及肾上主动脉收缩后血浆肾素活性的JAR研究。本实验中使用的6只杂种犬体重为12-22公斤。先前已经详细描述了制作非滤过性肾脏模型的技术(1)。简而言之，手术包括输尿管结扎和2小时的全肾缺血，之后狗通过腹膜透析维持4天。这些动物每天在水中摄入200毫升20%的葡萄糖，以弥补无意识的水分损失，并在一定程度上保持卡路里的摄入。除了输尿管结扎和肾脏缺血外，通过将肾脏与除肾动静脉外的所有组织连接分离，两个肾脏都被切断了神经。解剖切除肾血管外膜和所有可见神经，并将5%苯酚溶液涂抹在血管上。一圈聚乙烯管松散地绕着主动脉放置在右肾动脉起始处的前面。管子的两端穿过另一段大口径聚乙烯管子，并在距离伤口几厘米处的皮肤下缝合。术后第4天进行急性实验。取两份对照血样，用Statham压力传感器和SanBorn记录系统通过股动脉直接穿刺法测量血压。然后，狗被快速放血20ml/kg体重，并获得第二次血压测量。清醒的狗被允许不受干扰地躺在地板上，并在30、60和90分钟采集血样。在最后一次采样后，再次输血，允许狗恢复3小时。在此期间，将导管插入股动脉，并将导管顶端放置在松动的主动脉结扎的正后方。此时，主动脉结扎的游离端也暴露了出来。这两个小手术都是
MATERIALS AND METHODSDesign Jar study of plasma renin activity after hemorrhage and ajter suprarenal aortic constriction in dogs with deneruated, non-Jiltering kidneys. The six mongrel dogs used in this experiment weighed 12-22 kg. The technique for producing the nonfiltering kidney model has been previously described in detail (1). Briefly, the procedure involved ureteral ligation and a 2-hr period of total renal ischemia after which the dogs were maintained for 4 days by peritoneal dialysis. The animals received 200 ml of 20% dextrose in water daily to replace insensible water loss and to maintain to some extent caloric intake. In addition to ureteral ligation and renal ischemia, both kidneys were denervated by separating the kidney from all tissue connections except the renal arterv and vein. The adventitia of the renal vessels and all visible nerves were carefully removed by dissection and a 5% phenol solution applied to the vessels. A loop of polyethylene tubing was placed loosely around the aorta immediately anterior to the origin of the right renal artery. The ends of the tubing were passed through another length of large bore polyethylene tubing and sutured beneath the skin a few centimeters from the incision. On the 4th day after surgery the acute experiment was performed. Two control blood samples were obtained and blood pressure was measured by direct femoral artery puncture with a Statham pressure transducer and a Sanborn recording system. The dogs were then rapidly bled 20 ml/kg of body weight and a second blood pressure measurement was obtained. The conscious dogs were allowed to lie on the floor undisturbed and blood samples were obtained at 30, 60, and 90 min. After the last sampling period, the blood was reinfused and the dogs allowed to recover for 3 hr. During this period, a catheter was introduced into a femoral artery and the tip positioned to lie immediately posterior to the loose aortic ligature. The free ends of the aortic ligature were also exposed at this time. Both of these minor surgical procedures