Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion
Cross-linking of cell surface receptors enhances cooperativity of molecular adhesion
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DOI：
10.1016/s0006-3495(00)76824-x
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发表时间：
2000-06-01
影响因子：
3.4
通讯作者：
Moy, VT
中科院分区：
文献类型：
--
作者：
Chen, A;Moy, VT
分子粘附的协同性已被提出作为增强粘附分子在细胞表面上的结合强度的机制。利用原子力显微镜（Atomic force microscope，AFM）研究了偶联到AFM针尖上的刀豆球蛋白A（concanavalin A，Con A）与NIH 3 T3成纤维细胞表面Con A受体的粘附强度。受体与戊二醛或3，3 '-二硫代-双（磺基琥珀酰亚胺基丙酸酯）（DTSSP）的交联导致粘附增加，这可能归因于粘附复合物之间增强的协同性。由于固定细胞的更大刚度而导致的加载速率的增加也有助于结合强度的两倍增加。这些结果表明，受体交联可以通过产生向受体的合作结合的转变而大大有助于细胞粘附的总体增加。
Cooperativity of molecular adhesion has been proposed as a mechanism for enhanced binding strength of adhesion molecules on the cell surface. Direct evidence for its mechanism, however, has been lacking until now, Atomic force microscopy (AFM) was used to measure the adhesive strength between concanavalin A (Con A) coupled to an AFM tip and Con A receptors on the surface of NIH3T3 fibroblast cells. Cross-linking of receptors with either glutaraldehyde or 3,3'-dithio-bis(sulfosuccinimidylproprionate) (DTSSP) led to an increase in adhesion that could be attributed to enhanced cooperativity among adhesion complexes. An increase in loading rate due to greater stiffness of fixed cells also contributed to the twofold increase in binding strength. These results show that receptor cross-linking can greatly contribute to a total increase in cell adhesion by creating a shift toward cooperative binding of receptors.