The gene-expression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia
The gene-expression and phenotypic response of hFOB 1.19 osteoblasts to surface-modified titanium and zirconia
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DOI：
10.1016/j.biomaterials.2008.10.054
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发表时间：
2009-02-01
期刊：
影响因子：
14
通讯作者：
Kohal, Ralf J.
中科院分区：
文献类型：
--
作者：
Setzer, Bernhard;Baechle, Maria;Kohal, Ralf J.
具有增殖和分化潜力的成骨细胞系hFOB 1.19显示，细胞分化不受新开发的氧化锆植入物材料的材料和粗糙度的影响。研究材料为表面加工钛（Ti-m）、改性钛（TiUnite（R））、加工氧化锆（TZP-A-m）、改性氧化锆（ZiUnite（R））、加工氧化铝增韧氧化锆（ATZ-m）和改性氧化铝增韧氧化锆（AU-mod）。在通过扫描电子显微镜（SEM）和原子力显微镜（AFM）进行表面描述之后，在第1、3、7、14、21和28天之后检查细胞增殖（EZ 4U，Casy 1）和分化。通过碱性磷酸酶染色、矿化试验（茜素红）和骨和细胞外基质相关基因的表达分析（RT-PCR）观察成骨分化。钛和氧化锆在粗糙表面上的增殖减少。共聚焦激光扫描显微镜（CLSM）记录的细胞附着和细胞骨架组织阐明衰减的细胞附着在第一个4小时内受损的增殖的原因。在早期事件（RUNX 2、NELL-1、RUNX 3和BMP 7）和晚期事件（整联蛋白B3）中可以观察到TiUnite（R）和ZiUnite（R）的m-RNA的特异性上调。对于钛，IBSP和整联蛋白131的所有上调可在B1天描述。总之，分化既不受材料，也不粗糙。(C)2008爱思唯尔有限公司保留所有权利。
The osteoblastic cell-line hFOB 1.19 with the potential to proliferate and differentiate revealed that cellular differentiation is not affected by material and roughness on newly developed zirconia implant materials. Materials under investigation were surfaces machined titanium (Ti-m), modified titanium (TiUnite (R)), machined zirconia (TZP-A-m), modified zirconia (ZiUnite (R)), machined alumina-toughened zirconia (ATZ-m) and modified alumina-toughened zirconia (AU-mod). After surface description by scanning electron microscopy (SEM) and atomic force microscopy (AFM), cellular proliferation (EZ4U, Casy 1) and differentiation were examined after clays 1, 3, 7,14, 21, and 28. Osteogenic differentiation was visualized by alkaline phosphatase staining, mineralization assay (alizarin red) and by expression analysis (RT-PCR) of bone- and extracellular matrix-related genes. Proliferation on rough surfaces was reduced oil both titanium and zirconia. Cell-attachment and cytoskeleton organization documented by confocal laser scanning microscopy (CLSM) elucidated attenuated cell attachment within the first 4 h to be the reason for impaired proliferation. A specific up-regulation of m-RNAs in an early event (RUNX2, NELL-1, RUNX3, and BMP7) and a late event (Integrin B3) could be observed oil TiUnite (R) and ZiUnite (R). For titanium all up-regulation of IBSP and Integrin 131 Could be described at day B1. In total, differentiation was neither affected by material nor by roughness. (C) 2008 Elsevier Ltd. All rights reserved.