Extracts of Artemisia ciniformis Protect Cytotoxicity Induced by Hydrogen Peroxide in H9c2 Cardiac Muscle Cells through the Inhibition of Reactive Oxygen Species
Extracts of Artemisia ciniformis Protect Cytotoxicity Induced by Hydrogen Peroxide in H9c2 Cardiac Muscle Cells through the Inhibition of Reactive Oxygen Species
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DOI：
10.1155/2013/141683
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发表时间：
2013-01-01
影响因子：
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通讯作者：
Hosseinzadeh, Leila
中科院分区：
文献类型：
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作者：
Mojarrab, Mahdi;Jamshidi, Maryam;Hosseinzadeh, Leila
Objective.中华蒿（Artemisia ciniformis）和菊科蒿（A. biennis是伊朗自然生长的34种蒿属植物中的两种。本研究探讨了不同提取物对A. ciniformis和A. biennis对过氧化氢诱导的大鼠心肌细胞（H9 c2）的细胞毒性具有保护作用。法用石油醚（40-60）、二氯甲烷、乙酸乙酯（EA）、乙醇（EtOH）和乙醇：水（1：1）浸渍法分别提取这两种植物的干燥和粉碎的地上部分。评价不同提取物对A. ciniformis和A.为了研究山慈姑提取物对H_2O_2细胞毒性的保护作用，我们检测了H_2O_2在不同提取物存在和不存在下对H_9c_2细胞的直接细胞毒性作用。然后通过MTT法测定细胞活力。结果H_2O_2诱导的细胞毒性呈浓度依赖性。暴露24小时的IC 50值为62.5 μ M。然而，用不同浓度的EA、EtOH和EtOH/wt A. ciniformis保护细胞免受H2 O2诱导的细胞毒性。此外，用EA、EtOH和EtOH/wt提取物预处理A. ciniformis导致活性氧（ROS）产生的减少。综合以上结果，我们的观察表明，A. ciniformis对H2 O2诱导的H9 c2细胞毒性有保护作用。
Objective. Artemisia ciniformis (Asteraceae) and A. biennis are two of 34 Artemisia species growing naturally in Iran. In this study we investigated whether different extracts of A. ciniformis and A. biennis have protective effect against hydrogen peroxide-induced cytotoxicity in rat cardiomyoblast cells (H9c2). Method. The dried and ground aerial parts of these two species were extracted successively using petroleum ether (40-60), dichloromethane, ethyl acetate (EA), ethanol (EtOH) and ethanol : water (1 : 1) by maceration method. To evaluate whether different extracts of A. ciniformis and A. biennis protect cardiomyoblast H9c2 cells from H2O2 cytotoxicity, we examined the direct cytotoxic effect of H2O2 on H9c2 cells in the presence and absence of different extracts. After then, cell viability was measured by MTT assay. Results. H2O2 induced cytotoxicity in a concentration dependent manner. The IC50 value was 62.5 mu M for 24 h exposure. However, pretreatment of cells with various concentrations of EA, EtOH, and EtOH/wt extract of A. ciniformis protected cells from H2O2-induced cytotoxicity. Moreover, pretreatment with EA, EtOH and EtOH/wt extracts of A. ciniformis lead to a decrease in the reactive oxygen species (ROS) generation. Taken together our observation indicated that nontoxic concentration of different extracts of A. ciniformis has protective effect on H2O2-induced cytotoxicity in H9c2 cells.