A rapid derivatization based LC-MS/MS method for quantitation of short-chain fatty acids in human plasma and urine
A rapid derivatization based LC-MS/MS method for quantitation of short-chain fatty acids in human plasma and urine
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DOI：
10.4155/bio-2018-0241
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发表时间：
2019-04-01
期刊：
影响因子：
1.8
通讯作者：
Shahidi-Latham, Sheerin K.
中科院分区：
文献类型：
--
作者：
Jaochico, Allan;Sangaraju, Dewakar;Shahidi-Latham, Sheerin K.
目的：建立一种可靠的多基质LC-MS/MS定量测定人血浆和尿液中内源性短链脂肪酸（SCFA）生物标志物的方法。方法：该方法采用稳定同位素标记内标，高通量衍生化程序制备样品，并在正电喷雾电离模式下使用多反应监测转变进行LC-MS/MS分析。结果：采用替代基质法定量。准确度、精密度、平行度、曲线线性度、衍生化效率、稳定性和回收率均在可接受的标准范围内。结论：通过LC-MS/MS可以定量比较炎症性肠病患者与非疾病患者血浆和尿液中的SCFA水平。
Aim: Objective of this study is to develop a robust multi-matrix LC-MS/MS for the quantitation of endogenous short-chain fatty acids (SCFA) biomarkers in human plasma and urine. Methods: Developed method utilizes stable isotope-labeled internal standards, high-throughput derivatization procedure for sample preparation and LC-MS/MS analysis using multiple reaction monitoring transitions in positive electrospray ionization mode. Results: Surrogate matrix method was used for quantitation. Accuracy, precision, parallelism, curve linearity, derivatization efficiency, stability and recovery were all evaluated, and the results were well within the acceptable criteria. Conclusion: SCFA levels in human plasma and urine of inflammatory bowel disease patients versus non-disease subjects were quantified and compared by LC-MS/MS.