Direct demonstration of increased intracellular concentration of free calcium in rabbit and human neutrophils following stimulation by chemotactic factor.
Direct demonstration of increased intracellular concentration of free calcium in rabbit and human neutrophils following stimulation by chemotactic factor.
复制标题
直接证明在趋化因子刺激后，兔和人中性粒细胞的细胞内游离钙浓度增加。
DOI：
10.1016/0006-291x(83)90429-1
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
Sha'afi,RI
中科院分区：
文献类型：
--
作者：
White,JR;Naccache,PH;Molski,TF;Borgeat,P;Sha'afi,RI
用钙敏感的荧光探针quin-2直接证实了趋化因子刺激的兔和人中性粒细胞内游离钙浓度的增加。向中性粒细胞中加入f-Met-Leu-Phe（10− 9 M）、C5 a（3×10− 9 M）或白三烯B4（6 × 10− 8 M）可诱导细胞内游离钙浓度迅速增加，并在刺激后15秒达到最大值。当f-Met-Leu-Phe浓度低于10− 8 M时，增强作用呈剂量依赖性，ED 50为8 × 10− 11 M，并且在悬浮培养基中存在EGTA时显著降低。
An increase in the level of intracellular free calcium concentration in rabbit and human neutrophils stimulated by chemotactic factors has been demon-strated directly using the calcium-sensitive fluorescent probe quin-2. Addition of f-Met-Leu-Phe (10−9M), C5a(3×10−9M) or leukotriene B4(6 × 10−8M) to the neutrophils induces a rapid increase in the intracellular concentration of free calcium that reaches a maximum value 15 seconds following stimulation. At concentrations of f-Met-Leu-Phe less than 10−8M the enhancement is dose dependent with an ED50of 8 × 10−11M and is significantly reduced in the presence of EGTA in the suspending medium.