Development of AT1 and AT2 receptors in the ovine fetal brain
Development of AT1 and AT2 receptors in the ovine fetal brain
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DOI：
10.1016/j.devbrainres.2004.02.007
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发表时间：
2004-05-19
期刊：
影响因子：
--
通讯作者：
Xu, ZC
中科院分区：
文献类型：
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作者：
Hu, F;Morrissey, P;Xu, ZC
本研究采用蛋白质印迹法（Westernblot）、RT-PCR和免疫组织化学染色法，分别从蛋白质水平、mRNA水平和特异性受体免疫反应性三个方面，对早产至足月绵羊胎脑中AT和AT_2受体的发育进行了研究。结果表明，从早产到足月，胎羊脑中AT（1）和AT（2）受体的发育呈递增趋势。在0.7胎龄时，AT 1和AT 2受体均已出现在血管紧张素相关的中枢心血管和体液控制途径的主要结构中。重要的是，在胎儿下丘脑的视上核，以及在胎儿后脑的臂旁外侧核和延髓腹外侧区中已经发现了AT 1受体。这提供了血管紧张素受体在脑区的解剖学存在的证据，这些脑区对子宫内的心血管和液体调节功能至关重要。此外，虽然结果表明，AT 2受体在几个区域，如小脑在羊胎脑中的优势，占主导地位的占领AT，受体在下丘脑已出现在羊动物出生前的生命早期。总之，这些数据支持这样的假设，即中枢血管紧张素受体在妊娠的最后三个月发育良好并建立起来。脑受体为血管紧张素在维持子宫内胎儿生理功能（包括心血管和体液平衡）中的作用提供了药理学基础。(C)2004 Elsevier B. V.保留所有权利。
This study determined the development of AT, and AT, receptors in the ovine fetal brain from preterm to term by utilizing Western blot for the receptor expression at the protein level, RT-PCR for the receptor mRNA, and immunostaining for the specific receptor immunoreactivity. The results demonstrated that AT(1) and AT(2) receptors developed in an increasing pattern from preterm to term gestational periods in the fetal sheep brain. Both AT, and AT2 receptors have appeared in the major structures in the angiotensin-related central cardiovascular and body fluid controlling pathways at the 0.7 of the gestational age. Importantly, AT, receptors have been discovered in the supraoptic nuclei in the fetal hypothalamus, and in the lateral parabrachial nuclei and the ventrolateral medulla in the fetal hindbrain. This provides evidence of the anatomical existence of the angiotensin receptors in the brain areas that are critical for cardiovascular and fluid regulatory functions in utero. In addition, although the results demonstrated the predominance of AT2 receptors in several regions such as the cerebellum in the ovine fetal brain, dominant occupation of AT, receptors in the hypothalamus have appeared early in the life of sheep animals before birth. Together, the data support the hypothesis that the central angiotensin receptors are well developed and established in the last third trimester of gestation. The brain receptors provide a pharmacological basis for the action of angiotensin in the maintenance of in utero fetal physiological functions, including cardiovascular and body fluid balance. (C) 2004 Elsevier B.V. All rights reserved.