Molecular cloning, chromosomal location and expression pattern of porcine CIDEa and CIDEc
Molecular cloning, chromosomal location and expression pattern of porcine CIDEa and CIDEc
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猪CIDEa和CIDEc的分子克隆、染色体定位及表达模式
DOI：
10.1007/s11033-008-9216-5
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发表时间：
2009-03
影响因子：
2.8
通讯作者：
Zhou, L.
中科院分区：
文献类型：
--
作者：
Zhang, J.;Li, Y. H.;Feng, S. Q.;Lei, T.;Peng, Y.;Yang, Z. Q.;Chen, X. D.;Xia, T.;Long, Q. Q.;Zhou, L.
细胞死亡诱导DFF 45样效应子a和c（CIDEa和CIDEc）是新的凋亡诱导因子锡德家族的两个成员。除作为促凋亡蛋白外，已报道CIDEa和CIDEc可参与脂质或脂肪代谢。本研究首次报道了猪CIDEa和CIDEc的cDNA克隆、染色体定位和表达分析。序列分析表明，猪CIDEa基因开放阅读框为660 bp，编码219个氨基酸; CIDEc基因编码区为717 bp，编码238个氨基酸。猪CIDEa和CIDEc的推导氨基酸序列与其相应的人和小鼠同源物显示出高度相似性。辐射杂交图谱显示，猪CIDEA和CIDEC分别位于染色体6 q21 -26和13 q31，与人类染色体上相应同源物的基因座同线。组织分布分析表明，猪CIDEa和CIDEc mRNA在各种组织中共表达。两者均在白色脂肪组织中高表达，CIDEC mRNA在猪小肠、淋巴和脑中也有相对高水平的表达。此外，在白色脂肪组织和肝脏中，肥胖猪的CIDEa和CIDEc mRNA水平显著高于瘦猪。我们的研究为进一步研究这两个基因的功能提供了基础的分子信息，这将有助于更好地理解CIDEs在脂质代谢中的作用。
Cell death-inducing DFF45-like effectors a and c (CIDEa and CIDEc) are two members of the novel CIDE family of apoptosis-inducing factors. Except as pro-apoptotic proteins, it has been reported that CIDEa and CIDEc could be involved in lipid or fat metabolism. Here we first reported the cDNA cloning, chromosome mapping and expression analysis of CIDEa and CIDEc in pigs. Sequence analysis showed that porcine CIDEa contains an open reading frame of 660 bp, which encodes 219 amino acids, and CIDEc contains a coding region of 717 bp that encodes 238 amino acids. The deduced amino acid sequence of porcine CIDEa and CIDEc shows high similarities to their corresponding human and mouse homologues. Radiation hybrid mapping demonstrated that porcine CIDEa and CIDEc are located at chromosome 6q21-26 and 13q31 respectively, syntenic with the loci of their corresponding homologues on human chromosomes. Tissue distribution analysis indicated that porcine CIDEa and CIDEc mRNAs are co-expressed in various tissues. Both of them were highly expressed in white adipose tissue, and CIDEc mRNA was also expressed at relatively high level in porcine small intestine, lymph and brain. Furthermore, CIDEa and CIDEc mRNA level in white adipose tissues and liver were significantly higher in obese pigs than in their lean counterparts. Our studies provide basic molecular information useful for the further investigation on the function of the two genes, which will be helpful in better understanding of the roles of CIDEs in lipid metabolism.
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影响因子：
6.4
作者：
Marone M;Mozzetti S;De Ritis D;Pierelli L;Scambia G
通讯作者：
Scambia G
DOI：
10.1073/pnas.111145098
发表时间：
2001-05
影响因子：
11.1
作者：
P. Zhou;A. Lugovskoy;J. McCarty;Peng Li;G. Wagner
通讯作者：
P. Zhou;A. Lugovskoy;J. McCarty;Peng Li;G. Wagner
DOI：
10.1159/000134338
发表时间：
1996-01-01
期刊：
CYTOGENETICS AND CELL GENETICS
影响因子：
--
作者：
Yerle, M;Echard, G;Gellin, J
通讯作者：
Gellin, J
影响因子：
7.7
作者：
Nordström, EA;Rydén, M;Arner, P
通讯作者：
Arner, P
影响因子：
--
作者：
Paul Mickey Williams;D. Chang;Ulrich Danesch;Gordon M. Ringold;Renu A. Heller
通讯作者：
Paul Mickey Williams;D. Chang;Ulrich Danesch;Gordon M. Ringold;Renu A. Heller