Long non-coding RNA (lncRNA) MALAT1 in regulating osteogenic and adipogenic differentiation using a double-stranded gapmer locked nucleic acid nanobiosensor
Long non-coding RNA (lncRNA) MALAT1 in regulating osteogenic and adipogenic differentiation using a double-stranded gapmer locked nucleic acid nanobiosensor
复制标题
使用双链间隙体锁定核酸纳米生物传感器调节长非编码RNA (lncRNA) MALAT1 成骨和脂肪形成分化
DOI：
10.1039/d3an01531a
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Wang, Shue
中科院分区：
文献类型：
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作者：
Fasciano, Samantha;Luo, Shuai;Wang, Shue
长链非编码RNA（lncRNA）是长度超过200个核苷酸的非蛋白质编码RNA分子。lncRNA分子在基因调控、染色质重塑和细胞过程中起着不同的作用，影响着各种生物学途径。然而，探索lncRNA在活细胞中的复杂动力学是一项具有挑战性的任务。在这项研究中，双链gapmer锁核酸（ds-GapM-LNA）纳米生物传感器的设计用于可视化的丰度和表达的lncRNA在活的人骨髓间充质干细胞（hMSCs）。对该方法的敏感性、特异性和稳定性进行了表征。结果表明，这种ds-GapM-LNA纳米生物传感器具有非常好的灵敏度，特异性和稳定性，这允许解剖动态生理事件期间细胞过程的调节作用。通过将这种纳米生物传感器纳入活体hMSC成像，我们阐明了成骨和成脂分化过程中lncRNA MALAT 1的表达动态。数据显示，lncRNA MALAT 1表达与成骨和成脂分化的不同亚阶段相关。
Long non-coding RNAs (lncRNA) are non-protein coding RNA molecules that are longer than 200 nucleotides. The lncRNA molecule plays diverse roles in gene regulation, chromatin remodeling, and cellular processes, influencing various biological pathways. However, probing the complex dynamics of lncRNA in live cells is a challenging task. In this study, a double-stranded gapmer locked nucleic acid (ds-GapM-LNA) nanobiosensor is designed for visualizing the abundance and expression of lncRNA in live human bone-marrow-derived mesenchymal stem cells (hMSCs). The sensitivity, specificity, and stability were characterized. The results showed that this ds-GapM-LNA nanobiosensor has very good sensitivity, specificity, and stability, which allows for dissecting the regulatory roles of cellular processes during dynamic physiological events. By incorporating this nanobiosensor in living hMSC imaging, we elucidated lncRNA MALAT1 expression dynamics during osteogenic and adipogenic differentiation. The data reveal that lncRNA MALAT1 expression is correlated with distinct sub-stages of osteogenic and adipogenic differentiation.