Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera
Characterization of the two distinct subtypes of metabotropic glutamate receptors from honeybee, Apis mellifera
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DOI：
10.1016/j.neulet.2004.02.004
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发表时间：
2004-04-15
影响因子：
2.5
通讯作者：
Kadowaki, T
中科院分区：
文献类型：
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作者：
Funada, M;Yasuo, S;Kadowaki, T
L-谷氨酸是脊椎动物脑内兴奋性突触的主要神经递质。它也是昆虫神经肌肉接头的兴奋性神经递质，但其在大脑中的功能仍有待确定。我们确定并表征了两种不同的亚型（AmGluRA和AmGluRB）的代谢型谷氨酸受体（mGluRs）的真社会性昆虫，蜜蜂。AmGluRA和AmGluRB均在二硫键上独立形成同源二聚体，并分别以156.7和80.7 nM的K-D值结合[H-3]谷氨酸。AmGluRB在大脑中特异性表达，而AmGluRA在大脑和其他身体部位表达，这表明AmGluRA也存在于神经肌肉接头处。这两种mGluRs都在蜜蜂的蘑菇体和大脑区域表达，运动神经元聚集在那里。它们在大脑中的表达明显重叠，表明它们可能相互作用以调节多巴胺能神经传递。(C)2004爱思唯尔爱尔兰有限公司保留所有权利。
L-Glutamate is a major neurotransmitter at the excitatory synapses in the vertebrate brain. It is also the excitatory neurotransmitter at neuromuscular junctions in insects, however its functions in their brains remain to be established. We identified and characterized two different subtypes (AmGluRA and AmGluRB) of metabotropic glutamate receptors (mGluRs) from an eusocial insect, honeybee. Both AmGluRA and AmGluRB form homodimers independently on disulfide bonds, and bind [H-3]glutamate with K-D values of 156.7 and 80.7 nM, respectively. AmGluRB is specifically expressed in the brain, while AmGluRA is expressed in the brain and other body parts, suggesting that AmGluRA is also present at the neuromuscular junctions. Both mGluRs are expressed in the mushroom bodies and the brain regions of honeybees, where motor neurons are clustered. Their expression in the brain apparently overlaps, suggesting that they may interact with each other to modulate the glutamatergic neurotransmission. (C) 2004 Elsevier Ireland Ltd. All rights reserved.