Expression of Lyt-1 antigen on certain murine B cell lymphomas.
Expression of Lyt-1 antigen on certain murine B cell lymphomas.
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DOI：
10.1084/jem.153.4.998
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发表时间：
1981-04-01
期刊：
影响因子：
--
通讯作者：
Herzenberg LA
中科院分区：
文献类型：
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作者：
Lanier LL;Warner NL;Ledbetter JA;Herzenberg LA
虽然Lyt-1抗原以前被认为是T细胞特异性标记，但最近的证据表明在正常淋巴组织中存在Thy-1-、Lyt-1+细胞群.在这项研究中，我们观察到WEHI-55、WEHI-259和CH 5 B细胞淋巴瘤表达高水平的Lyt-1抗原，如使用荧光激活细胞分选仪通过单克隆抗体检测到的。另外三个B细胞淋巴瘤的11个检查也给出了弱，但可检测的反应与抗Lyt-1单克隆抗体。除了Lyt-1抗原的表达外，这些淋巴瘤在表面表型方面是典型的B细胞谱系细胞。代谢标记的WEHI-55细胞免疫沉淀的Lyt-1糖蛋白在结构上与胸腺细胞上的Lyt-1糖蛋白相似。这些发现类似于最近的报道，B型人类淋巴细胞白血病细胞表达Lyt-1的推定人类同源物，Leu-1抗原。
Although the Lyt-1 antigen has previously been considered a T cell- specific marker, recent evidence suggests that a population of Thy-1-, Lyt-1+ cells exists in normal lymphoid tissues. In this study, we have observed that the WEHI-55, WEHI-259, and CH5 B cell lymphomas express high levels of the Lyt-1 antigen, as detected by monoclonal antibodies using the fluorescence activated cell sorter. Three other B cell lymphomas of the 11 examined also gave weak but detectable reactions with the anti Lyt-1 monoclonal antibody. Except for the expression of the Lyt-1 antigen, these lymphomas are typical of cells in the B cell lineage with respect to surface phenotype. The Lyt-1 glycoprotein immunoprecipitated from metabolically labeled WEHI-55 cells is similar in structure to the Lyt-1 glycoprotein on thymocytes. These findings are similar to recent reports that B-type human lymphocytic leukemia cells express the putative human homologue of Lyt-1, the Leu-1 antigen.