FUNCTIONAL-SIGNIFICANCE AND EVOLUTIONARY DEVELOPMENT OF THE 5'-TERMINAL REGIONS OF IMMUNOGLOBULIN VARIABLE-REGION GENES
FUNCTIONAL-SIGNIFICANCE AND EVOLUTIONARY DEVELOPMENT OF THE 5'-TERMINAL REGIONS OF IMMUNOGLOBULIN VARIABLE-REGION GENES
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DOI：
10.1016/0092-8674(82)90184-2
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发表时间：
1982-01-01
期刊：
影响因子：
64.5
通讯作者：
PERRY, RP
中科院分区：
文献类型：
--
作者：
KELLEY, DE;COLECLOUGH, C;PERRY, RP
κ的5“-末端序列。通过分析两种κ基因，明确地确定在MPC 11浆细胞瘤细胞中表达的L链基因。mRNA和转录的基因。帽位点和翻译起始密码子之间的距离仅为3个核苷酸，并且在较小的变体中仅为2个核苷酸，远小于在其他种类的核衍生mRNA中发现的距离。用MPC 11核RNA进行的S1核酸酶保护实验表明，帽位点与转录起始位点一致，表明5“-末端异质性是由转录起始的不精确性引起的。MPC 11 V. κ的5“-末端结构的比较。与其他几个V基因的比较表明，由5“非翻译区和编码大部分信号肽的序列组成的第一外显子的长度是高度保守的。在这组实施例中，5“非翻译区从3-33个核苷酸变化，信号肽编码区从46-76个核苷酸变化。该分析还提供了对MPC 11 L链的2个异常性质的遗传起源的洞察。
The 5''-terminal sequence of the .kappa. L chain gene expressed in MPC11 plasmacytoma cells was definitively determined by analysis of both the .kappa. mRNA and the gene from which is is transcribed. The distance between the cap site and the translation initiation codon is only 3 nucleotides, and in a minor variant only 2 nucleotides, considerably less than that found in other species of nucleus-derived mRNA. S1 nuclease protection experiments with MPC11 nuclear RNA indicate that the cap sites are coincident with the transcriptional start sites, suggesting that the 5''-terminal heterogeneity is caused by imprecision in transcriptional initiation. A comparison of the 5''-terminal structure of the MPC11 V.kappa. gene with that of several other V genes indicates that the length of the 1st exon, which is composed of the 5'' untranslated region and a sequence encoding most of the signal peptide, is highly conserved. Within this set of examples, the 5'' untranslated region varies from 3-33 nucleotides, and the signal-peptide-coding block from 46-76 nucleotides. The analysis has also provided insight into the genetic origins for 2 anomalous properties of the MPC11 L chain.