Mesenchymal stem cell transplantation can restore lupus disease-associated miRNA expression and Th1/Th2 ratios in a murine model of SLE.
Mesenchymal stem cell transplantation can restore lupus disease-associated miRNA expression and Th1/Th2 ratios in a murine model of SLE.
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DOI：
10.1038/srep38237
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发表时间：
2016-12-07
影响因子：
4.6
通讯作者：
Kim SJ
中科院分区：
文献类型：
--
作者：
Choi EW;Lee M;Song JW;Shin IS;Kim SJ
C3。MRL-Faslpr/J小鼠自发出现高滴度的抗dsdna，轻度肾小球肾炎和严重的淋巴细胞增生症状。本研究旨在比较长期连续给药人脂肪组织源性间充质干细胞（ASCs）和环磷酰胺治疗C3的效果。MRL-Faslpr/J小鼠使用小鼠SLE模型。C3。MRL-Faslpr/J小鼠分为生理盐水(C)、环磷酰胺(Y)和ASC (H)治疗组。对照组C3H小鼠经生理盐水(N)处理后也进行了比较。Y组疾病参数改善最大，但小梁完整性受损。ASC移植可显著降低抗dsdna水平、肾小球C3沉积和CD138比例，且无小梁损伤。此外，环磷酰胺和ASC处理均显著降低了Th1/Th2比。C组miR-31-5p、miR-96-5p、miR-182-5p、miR-183-5p、miR-379-5p的表达水平显著高于N组，mir - 150-5p的表达水平显著低于N组。Y组和H组miR-96-5p、miR-182-5p的表达水平均显著低于C组。因此，环磷酰胺或ASC治疗可以改变mirna，降低miR-96-5p和miR-182-5p的表达，降低CD138比例和Th1/Th2比例，这可能与治疗机制有关。
C3.MRL-Faslpr/J mice spontaneously develop high titers of anti-dsDNA, mild glomerular nephritis, and severe lymphoproliferation symptoms. This study aimed to compare the effects of long-term serial administration of human adipose tissue-derived mesenchymal stem cells (ASCs), and cyclophosphamide treatment in C3.MRL-Faslpr/J mice using a murine SLE model. C3.MRL-Faslpr/J mice were divided into saline (C), cyclophosphamide (Y), and ASC (H) treatment groups. Background-matched control C3H mice treated with saline (N) were also compared. The Y group showed the greatest improvement in disease parameters, but with damaged trabecular integrity. ASC transplantation reduced anti-dsDNA levels, glomerular C3 deposition and CD138 proportion significantly, without trabecular damage. Furthermore, both cyclophosphamide and ASC treatment significantly decreased the ratio of Th1/Th2 compared with the saline-treatment. The expression levels of miR-31-5p, miR-96-5p, miR-182-5p, miR-183-5p, and miR-379-5p were significantly higher, while those of miR150-5p were significantly lower in the C group than in the N group. The expression levels of miR-96-5p, miR-182-5p in the Y and H groups were significantly lower than in the C group. Thus, treatment with cyclophosphamide or ASC can change miRNAs and decrease miR-96-5p and miR-182-5p expression, as well as decreasing the CD138 proportion and the Th1/Th2 ratio, which might be involved in the therapeutic mechanism.
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影响因子：
13.6
作者：
Crispín JC;Liossis SN;Kis-Toth K;Lieberman LA;Kyttaris VC;Juang YT;Tsokos GC
通讯作者：
Tsokos GC
影响因子：
4.4
作者：
Lacotte, Stephanie;Dumortier, Helene;Muller, Sylviane
通讯作者：
Muller, Sylviane
DOI：
10.1073/pnas.0808263106
发表时间：
2009-02-10
影响因子：
11.1
作者：
Segura, Miguel F.;Hanniford, Douglas;Hernando, Eva
通讯作者：
Hernando, Eva
影响因子：
--
作者：
Sadanaga, Atsushi;Nakashima, Hitoshi;Harada, Mine
通讯作者：
Harada, Mine
影响因子：
4
作者：
Choi, Eun Wha;Shin, Il Seob;Seong, Je Kyung
通讯作者：
Seong, Je Kyung