Thermodynamic Complexation Mechanism of Zinc Ion with 8-Hydroxyquinoline-5-Sulfonic Acid in Molecular Crowding Environment
Thermodynamic Complexation Mechanism of Zinc Ion with 8-Hydroxyquinoline-5-Sulfonic Acid in Molecular Crowding Environment
复制标题
DOI：
10.1016/j.molliq.2022.121181
复制
发表时间：
2022-12
影响因子：
6
通讯作者：
Akihisa Miyagawa;Hiroyuki Komatsu;S. Nagatomo;K. Nakatani
中科院分区：
文献类型：
--
作者：
Akihisa Miyagawa;Hiroyuki Komatsu;S. Nagatomo;K. Nakatani
与本体溶液相比，在生物细胞中普遍观察到的拥挤的分子环境改变了生化反应的热力学和动力学。虽然分子拥挤效应受到了广泛的关注，但基于热力学参数（包括焓变（ΔH）和熵变（ΔS））的分子拥挤效应的基本解释是不够的。在分子拥挤环境中，基于ΔHand ΔS的变化，研究了锌离子与羟基喹啉衍生物在聚乙二醇（PEG）作用下的络合机理.对于1：1络合，由于脱水分子的数目减少，ΔHand Δ S随PEG浓度（CPEG）的增加而减小。在受渗透压和体积排阻效应影响的1：2络合体系中，ΔHand Δ SonCPEG没有明显的依赖性。这是因为渗透压和体积排阻效应对ΔHand ΔS的贡献相反。本文提供的结果为蛋白质-金属相互作用提供了基础知识，预计这些数据将吸引许多物理化学家和生物化学家的注意。
A crowded molecular environment, which is universally observed in biological cells, changes the thermodynamics and kinetics of biochemical reactions compared to bulk solution. Although molecular crowding has received significant attention, a fundamental explanation of the molecular crowding effect based on thermodynamic parameters, including enthalpy (ΔH) and entropy changes (ΔS), is insufficient. Herein, the complexation mechanism of zinc ions with quinolinol derivative in a molecular crowding environment was investigated using polyethylene glycol (PEG) based on the changes in ΔHand ΔS. For 1:1 complexation, which is only promoted by the osmotic pressure effect, ΔHand ΔSdecreased with increasing PEG concentration (CPEG) because the number of dehydration molecules decreased. In the 1:2 complexation system, which is affected by the osmotic pressure and volume exclusion effects, no obvious dependences of ΔHand ΔSonCPEGwere observed. This is because the osmotic pressure and volume exclusion effects contributed oppositely to ΔHand ΔS. The results presented herein provide fundamental knowledge for protein-metal interactions, and it is expected that these data will attract the attention of many physical chemists and biochemists.