Soyasaponin Ab protects against oxidative stress in HepG2 cells via Nrf2/HO-1/NQO1 signaling pathways
Soyasaponin Ab protects against oxidative stress in HepG2 cells via Nrf2/HO-1/NQO1 signaling pathways
复制标题
大豆皂苷 Ab 通过 Nrf2/HO-1/NQO1 信号通路保护 HepG2 细胞免受氧化应激
DOI：
10.1016/j.jff.2018.03.037
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发表时间：
2018-06-01
影响因子：
5.6
通讯作者：
Xie, Tianyu
中科院分区：
文献类型：
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作者：
Liu, Xiuying;Chen, Keke;Xie, Tianyu
黄芪皂苷Ab（SA）是从大豆中分离得到的一种典型的齐墩果烷型三萜皂苷，具有抗氧化和抗炎作用。本研究发现SA通过核因子红细胞2相关因子2（Nrf 2）信号通路刺激HepG 2细胞中某些II相解毒酶的表达。荧光素酶测定结果表明，SA通过与抗氧化反应元件结合来促进Nrf 2转录。荧光素酶法和Western blotting结合分析表明，SA激活了ERK 1/2信号通路，使Nrf 2从细胞质转位到细胞核。SA还上调了一些Nrf 2相关的抗氧化酶，如血红素加氧酶-1（HO-1）、NAD（P）H醌氧化还原酶-1（NQO 1）和谷氨酸半胱氨酸连接酶催化亚基（GCLC）。抑制si-Nrf 2抵抗SA诱导的抗氧化基因的表达。总的来说，这些结果表明，SA通过Nrf 2/HO-1/NQO 1信号通路发挥其对氧化应激的保护作用。
Soyasaponin Ab (SA) is a typical oleanane triterpene saponin isolated from soybean and represents effects of antioxidation and anti-inflammation. In this study, we found the expressions of some phase II detoxifying enzymes were stimulated by SA via the signaling pathway of nuclear factor erythroid 2-related factor 2 (Nrf2) in HepG2 cells. Luciferase assay results demonstrated that SA promoted the Nrf2 transcription via binding to the antioxidant response element. In addition, a combining of luciferase assay and Western blotting suggested that SA activated ERK1/2 signaling pathways, and made Nrf2 translocated from cytoplasm to nucleus. SA also up regulated some Nrf2-related antioxidant enzymes such as heme oxygenase-1 (HO-1), NAD(P)H quinone oxidoreductase-1 (NQO1), and glutamate cysteine ligase catalytic subunit (GCLC). Inhibition of si-Nrf2 resisted expressions of SA-induced antioxidant genes. Collectively, these results suggest that SA exerted its protection against oxidative stress via the Nrf2/HO-1/NQO1 signaling pathways.