Distribution of microtubule-associated proteins (MAPs) in adult and embryonic mouse retinal explants: presence of the embryonic map, MAP5/1B, in regenerating adult retinal axons.
Distribution of microtubule-associated proteins (MAPs) in adult and embryonic mouse retinal explants: presence of the embryonic map, MAP5/1B, in regenerating adult retinal axons.
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微管相关蛋白 (MAP) 在成年和胚胎小鼠视网膜外植体中的分布：胚胎图谱 MAP5/1B 在再生成年视网膜轴突中的存在。
DOI：
10.1006/dbio.1993.1050
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发表时间：
1993
影响因子：
2.7
通讯作者：
Meyer,RL
中科院分区：
文献类型：
--
作者：
Bates,CA;Trinh,N;Meyer,RL
微管相关蛋白(MAP)是参与微管聚合和稳定的发育调节蛋白。我们问过再生的成年小鼠视网膜神经节细胞轴突的图谱是否概括了发育过程中所看到的图谱模式。成年和胚胎第15天(E15)小鼠的视网膜片段被移植到涂有层粘连蛋白的盖片上，并允许在无血清培养液中延伸神经突起。用多种单抗对成体和E15外植体培养的早期(MAP5)和晚期(MAP1、MAP2、MAP2a+b、tau)图谱进行染色。E15外植体长出的视神经突起对MAP呈强阳性反应。MAP1和MAP2a+b没有标记，MAP2和tau的单抗对轴突和胞体的染色较浅，可能是由于MAP2c和幼年tau在发育中的神经元中发现的。在成体外植体培养中，核膜和轴突均呈MAP1免疫反应阳性。MAP2和MAP2a+b标记外植体内的核膜和纤维，tau染色底物上的轴突。在成年外植体培养中，MAP5免疫反应强烈，标记的轴突延伸到基质和核周膜以及外植体中的树突，其模式类似于E15外植体。综上所述，成年视网膜外植体维持其成体MAP的补充，表现出高水平的MAP1，tau免疫反应仅限于基质上的轴突，MAP2分离到外植体内的轴突和核周。例外的是MAP5，其免疫反应模式在成体和E15视网膜外植体中相似。
Microtubule-associated proteins (MAPs) are developmentally regulated proteins involved in microtubule polymerization and stabilization. We have asked whether MAPs in regenerating adult mouse retinal ganglion cell axons recapitulate the pattern of MAPs seen during development. Adult and Embryonic Day 15 (E15) mouse retinal segments were explanted onto laminin-coated coverslips and allowed to extend neurites in serum-free medium. Using a variety of monoclonal antibodies (Mabs), we stained adult and E15 explant cultures for early (MAP5) and late (MAP1, MAP2, MAP2a+b, tau) MAPs. Optic neurites growing from E15 explants were strongly immunoreactive for MAPS. Labeling was absent for MAP1 and MAP2a+b. Mabs to MAP2 and tau lightly stained axons and cell bodies, probably due to MAP2c and juvenile tau which are found in developing neurons. In adult explant cultures, both perikarya and axons were immunoreactive for MAP1. MAP2 and MAP2a+b labeled perikarya and fibers within the explant, and tau stained axons on the substrate. MAP5 immunoreactivity was intense in the adult explant cultures, labeling axons extending over the substrate and perikarya and dendrites in the explant in a pattern similar to that seen in E15 explants. In summary, adult retinal explants maintain their adult complement of MAPs, in showing high levels of MAP1, tau immunoreactivity restricted to neurites on the substrate, and MAP2 segregated into neurites and perikarya within the explant. The exception is MAP5 for which the pattern of immunoreactivity is similar in adult and E15 retinal explants.