Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins
Molecular cloning and characterization of mammalian homologues of vesicle-associated membrane protein-associated (VAMP-associated) proteins
复制标题
DOI：
10.1006/bbrc.1998.9876
复制
发表时间：
1999-01-08
影响因子：
3.1
通讯作者：
Tanaka, T
中科院分区：
文献类型：
--
作者：
Nishimura, Y;Hayashi, M;Tanaka, T
我们已经鉴定了人和大鼠的VAMP相关蛋白（VAP）的33 kDa的加州无尾吸虫（aVAP-33），我们指定VAP-A，VAP-B，和VAP-C的同源物。发现人VAP-A（hVAP-A）与最近报道的蛋白质hVAP-33相同，除了两个氨基酸残基之外。VAP-B含有卷曲螺旋结构域和跨膜结构域（TMD）。人VAP-B（hVAP-B）分别与aVAP-33和hVAP-A在氨基酸水平上有46%和60%的同源性。人VAP-C是hVAP-B的剪接变体，缺乏卷曲螺旋结构域和TMD。hVAP-B具有与VAMP结合的能力。此外，hVAP-A和hVAP-B通过各自的TMD相互结合。这些结果表明，VAP形成复合物可能在哺乳动物囊泡的运输中起重要作用。(C)北京：科学出版社.
We have identified human and rat homologues of the VAMP-associated protein (VAP) of 33 kDa of Aplysia californica (aVAP-33), which we designated VAP-A, VAP-B, and VAP-C. Human VAP-A (hVAP-A) was found to be identical to the recently reported protein hVAP-33, with the exception of two amino acid residues. VAP-B contained a coiled-coil domain and a transmembrane domain (TMD). Human VAP-B (hVAP-B) was 46 and 60% homologous of the amino acid level to aVAP-33 and hVAP-A, respectively. Human VAP-C was a splicing variant of hVAP-B, lacking both the coiled-coil domain and the TMD. hVAP-B had VAMP-binding ability. Moreover, hVAP-A and hVAP-B associated with each other through their respective TMDs, These results suggest that complex formation by VAPs might be important in the trafficking of mammalian vesicle. (C) 1999 Academic Press.