The role of caspase 3 in the mud crab (Scylla paramamosain) after Vibrio parahaemolyticus infection.
The role of caspase 3 in the mud crab (Scylla paramamosain) after Vibrio parahaemolyticus infection.
复制标题
DOI：
10.1016/j.fsi.2021.09.010
复制
发表时间：
2021-09
影响因子：
4.7
通讯作者：
Changhong Cheng;Xiucheng Liu;Hongling Ma;Guangfeng Liu;Yiqin Deng;Juan Feng;Yu-Kun Jie;Zhixun Guo
中科院分区：
文献类型：
--
作者：
Changhong Cheng;Xiucheng Liu;Hongling Ma;Guangfeng Liu;Yiqin Deng;Juan Feng;Yu-Kun Jie;Zhixun Guo
细胞凋亡在机体抵抗病原体感染的免疫防御机制中起重要作用。半胱天冬酶3是参与细胞凋亡和免疫应答的半胱氨酸蛋白酶家族。本研究克隆了拟穴青蟹（Scylla paramamosain）caspase 3的全长基因（命名为Sp-caspase 3）。Sp-caspase 3的开放阅读框为1035 bp，编码344个氨基酸。SP-caspase 3在多种组织中广泛表达，在血细胞中高水平表达。细胞定位分析显示，Sp-caspase 3定位于细胞质和细胞核。Sp-caspase 3过表达可诱导细胞凋亡。此外，副溶血弧菌感染诱导caspase-3 mRNA的相对表达，并使caspase-3活性增加。体内敲低Sp-caspase 3可显著减少V.副溶血感染这些结果表明Sp-caspase 3在机体抗细菌感染的免疫应答和细胞凋亡中起重要作用。
Apoptosis plays essential roles in the immune defense mechanism against pathogen infection. Caspase 3 is a family of cysteine proteases involved in apoptosis and the immune response. In this study, the full-length of mud crab (Scylla paramamosain) caspase 3 (designated as Sp-caspase 3) was cloned and characterized. The open reading frame of Sp-caspase 3 was comprised a 1035 bp, which encoded a putative protein of 344 amino acids. Sp-caspase 3 was ubiquitously expressed in various tissues with a high-level expression in hemocytes. Cellular localization analysis revealed that Sp-caspase 3 was located in the cytoplasm and nucleus. Over-expression of Sp-caspase 3 could induce cell apoptosis. In addition,V. Parahaemolyticusinfection induced the relative expression of caspase-3 mRNA and increased caspase-3 activity. Knocking down Sp-caspase 3 in vivo significantly reduced cell apoptosis and increased mortality of mud crab afterV. parahaemolyticusinfection. These results indicated that Sp-caspase 3 played important roles in the immune response and apoptosis against bacterial infection.