Cross-protection against a lethal influenza virus infection by DNA vaccine to neuraminidase
Cross-protection against a lethal influenza virus infection by DNA vaccine to neuraminidase
复制标题
DOI：
10.1016/s0264-410x(00)00149-3
复制
发表时间：
2000-08-01
期刊：
影响因子：
5.5
通讯作者：
Tamura, S
中科院分区：
文献类型：
--
作者：
Chen, Z;Kadowaki, S;Tamura, S
用不同亚型a病毒编码神经氨酸酶（NA）的质粒dna免疫BALB/c小鼠，观察其对致死性流感病毒感染的交叉保护作用。每个NA-DNA给药两次，间隔3周，每只小鼠1 μ g的剂量通过颗粒介导的DNA转移到表皮（基因枪）或每只小鼠30 μ g的剂量通过电穿孔进入肌肉。在第二次接种疫苗三周后，用致死剂量的同源或异源病毒攻击小鼠，并通过测定肺病毒滴度、体重和存活率来评估每种NA-DNA保护小鼠免受流感侵害的能力。H3N2病毒的NA-DNA对同一亚型内的抗原变异的致命攻击具有交叉保护作用，但不能对不同亚型病毒（H1N1）的感染提供保护。对感染的交叉保护程度与交叉反应抗体的滴度有关。这些结果表明，dna - dna可以作为疫苗成分，提供有效的保护，不仅与同源病毒感染，也与漂移病毒感染。(C) 2000 Elsevier Science Ltd.版权所有。
Cross-protection against a lethal influenza virus infection was examined in BALB/c mice immunized with plasmid DNAs encoding the neuraminidase (NA) from different subtype A viruses. Each NA-DNA was administered twice, 3 weeks apart, at the dose of 1 mu g per mouse by particle-mediated DNA, transfer to the epidermis (gene gun) or at a dose of 30 mu g per mouse by electroporation into the muscle. Three weeks after the second vaccination, the mice were challenged with lethal doses of homologous or heterologous viruses and the ability of each NA-DNA to protect the mice from influenza was evaluated by determining the lung virus titers, body weight and survival rates. The H3N2 virus NA-DNA conferred cross-protection against lethal challenge with antigenic variants within the same subtype, but failed to provide protection against infection by a different subtype virus (H1N1). The degree of cross-protection against infection was related to titers of the cross-reacting antibodies. These results suggest that NA-DNA can be used as a vaccine component to provide effective protection against infection not only with homologous virus but also with drift viruses. (C) 2000 Elsevier Science Ltd. All rights reserved.