Hepatic lipid droplet breakdown through lipolysis during hibernation in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
Hepatic lipid droplet breakdown through lipolysis during hibernation in Chinese Soft-Shelled Turtle (Pelodiscus sinensis)
复制标题
中国鳖 (Pelodiscus sinensis) 冬眠期间肝脏脂滴通过脂肪分解而分解
DOI：
10.18632/aging.101887
复制
发表时间：
2019-04-15
期刊：
影响因子：
5.2
通讯作者：
Chen, Qiusheng
中科院分区：
文献类型：
--
作者：
Huang, Yufei;Chen, Hong;Chen, Qiusheng
Hibernation是一种适应寒冷和无食冬季的适应性生存策略。为了确定季节性适应的潜在机制，已经在熊，松鼠和蝙蝠中进行了转录组测序研究。尽管在确定与代谢有关的差异表达基因方面取得了进步，但这些生理适应性的确切机制尚不清楚。在本研究中，我们检查了中国软壳海龟（pelodiscus sinensis）的肝脏，发现在冬眠期间，脂质液滴（LD）和甘油三酸酯（TG）的含量显着降低。在冬眠过程中，与脂解相关基因的mRNA表达水平升高，脂肪生成相关基因的水平降低表明在冬眠过程中刺激了LD水解。为了从LD连续释放脂肪酸（FAS），募集脂肪甘油三酸酯脂肪酶（ATGL），并通过激活环状腺苷单磷酸腺苷（CAMP）/蛋白激酶A（PKA）信号来积聚在LDS表面上。同时，通过比较基因鉴定-58（CGI-58）和ATGL之间的相互作用，与LD相关蛋白（Perilipin-5）的磷酸化增加激活了ATGL的活性。综上所述，我们的结果表明，在冬眠期间，ATGL在LD表面及其诱导的酶活性促进了中国软壳龟（Pelodiscus sinensis）的LD分解，从而增强了线粒体β-氧化氧化的能量止血。
Hibernation is an adaptive survival strategy in response to cold and foodless winter. To determine the underlying mechanisms of seasonal adaptions, transcriptome sequencing studies have been conducted in bears, ground squirrels and bats. Despite advances in identifying differentially expressed genes involved in metabolism, the precise mechanisms of these physiological adaptions remain unclear. In the present study, we examined liver of Chinese Soft-Shelled Turtle (Pelodiscus sinensis) and found that the contents of lipid droplet (LD) and triglyceride (TG) were significantly decreased during hibernation. Increases in mRNA expression levels of lipolysis-related genes and decreased levels of lipogenesis-related genes during hibernation indicated that LD hydrolysis was stimulated during hibernation. To continuously release fatty acids (FAs) from LD, adipose triglyceride lipase (ATGL) was recruited and accumulated on the surface of LDs via activation of Cyclic Adenosine monophosphate (cAMP)/protein kinase A (PKA) signaling. Meanwhile, increased phosphorylation of the LD-associated protein, perilipin-5, activated the enzyme activity of ATGL via interaction between comparative gene identification-58 (CGI-58) and ATGL. Taken together, our results indicated that ATGL accumulation on the LD surface and its induced enzyme activity during hibernation promoted LD breakdown in the liver of Chinese Soft-Shelled Turtle (Pelodiscus sinensis), thereby enhancing mitochondrial beta-oxidation to maintain energy hemostasis.