Poster Session: Optical coherence elastography for In situ measurement of stiffness increase in posterior sclera after crosslinking.
Poster Session: Optical coherence elastography for In situ measurement of stiffness increase in posterior sclera after crosslinking.
复制标题
海报会议：光学相干弹性成像用于原位测量交联后后巩膜硬度增加。
DOI：
10.1167/jov.23.11.73
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发表时间：
2023
影响因子：
1.8
通讯作者：
Yun,Seok-Hyun
中科院分区：
文献类型：
--
作者：
Vinas-Pena,Maria;Feng,Xu;Li,Guoyang;Yun,Seok-Hyun
在眼睛生长过程中，巩膜的发育决定了眼睛的大小，从而决定了眼睛的屈光状态。近视的巩膜重塑包括巩膜变薄、巩膜组织丢失和力学性能减弱。因此，旨在硬化巩膜组织的干预（交联，SCXL）可能提供一种预防或治疗近视的方法。SCXL的发展需要工具来评估交联对组织力学性能的影响，特别是在巩膜中，与角膜相比，巩膜的力学性能在空间上更不均匀，各向异性，并且从前到后区域局部变化。在这里，我们应用高频OCE技术来测量后巩膜组织的不均匀力学特性，并评估SCXL后剪切模量的变化。作为模型系统，我们使用离体猪眼和核黄素辅助紫外交联。从测量的弹性波速（6-16kHz）来看，正常巩膜的平均面外剪切模量为0.71±0.12 MPa （n= 20）。处理后，剪切模量增加到1.50±0.39 MPa。这2倍的变化与静态杨氏模量从5.5±增加一致。交联后1 ~ 9.3±1.9 MPa，采用常规单轴伸测法。OCE显示了颞、鼻和更深后巩膜的区域刚度差异，证明了其作为一种无创工具测试巩膜交联效果的潜力。
During eye growth, scleral development critically determine eye size and thus the refractive status of the eye. Scleral remodeling in myopia includes scleral thinning, loss of scleral tissue, and weakening of the mechanical properties. Therefore, an intervention aiming at stiffening scleral tissues (crosslinking, SCXL) may provide a way to prevent or treat myopia. The development of SCXL requires tools to evaluate the effects of crosslinking on the mechanical properties of tissues, particularly in sclera where the mechanical properties are more spatially heterogeneous than in the cornea, anisotropic, and varying locally from the anterior to posterior regions. Here, we apply the high-frequency OCE technique to measure the heterogeneous mechanical properties of posterior scleral tissues and, evaluate the changes in shear moduli after SCXL. As a model system, we use ex vivo in porcine eyes and riboflavin-assisted UV crosslinking. From measured elastic wave speeds (6-16kHz), the average out-of-plane shear modulus was 0.71±0.12 MPa (n= 20) for normal scleras. After treatment, the shear modulus increased to 1.50±0.39 MPa. This 2-fold change was consistent with the increase of static Young’s modulus from 5.5±. 1 to 9.3±1.9 MPa after crosslinking, using conventional uniaxial extensometry. OCE revealed regional stiffness differences across the temporal, nasal, and deeper posterior sclera, demonstrating its potential as a noninvasive tool to test the effect of scleral crosslinking.