The role of Ulva fasciata in the evolution of the microbial community and antibiotic resistance genes in maricultural sediments.
The role of Ulva fasciata in the evolution of the microbial community and antibiotic resistance genes in maricultural sediments.
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DOI：
10.1016/j.marpolbul.2020.111940
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发表时间：
2020-12
影响因子：
5.8
通讯作者：
Yuqin Luo;Lijuan Feng;Guang-feng Yang;J. Mu
中科院分区：
文献类型：
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作者：
Yuqin Luo;Lijuan Feng;Guang-feng Yang;J. Mu
研究了不同密度(0、5、12 mg L−1)的褐藻与海水养殖文蛤共培养3个月后沉积物中微生物群落和抗生素抗性基因(ARGs)的变化。对−-N、PO43-−-P的最大去除速率和对弧菌培养能力的抑制均出现在L−1U.从原始生态位分离后，不含扁藻的对照底泥ARG总量显著减少14.0%，而添加5和12 g的L−1后，与对照底泥相比，ARGs总量分别增加了5.58%和4.65%，与寄主绿藻、螺旋藻、变形杆菌和拟杆菌密切相关。此外，法氏不动杆菌还抑制了一些四环素耐药基因的绝对基因数的下降，包括四环素耐药基因(TETPB、TETW、OTRA、TETI、TETO)和第一类整合子整合酶基因。
This study explored changes in the microbial community and antibiotic resistance genes (ARGs) in maricultural clam sediment after 3-month co-culture with different densities (0, 5 and 12 g L−1) of seaweedUlva fasciata(U.fasciata). The maximum removal rates of NO3−-N, PO43−-P, and inhibition ofVibrioculturability occurred at presence of 12 g L−1U.fasciata. A significant decrease by 14.0% of the total ARGs was found in control sediment withoutU.fasciataafter separation from the original niches, while the total ARGs further increased by 5.58%and 4.65% at presence of 5 and 12 g L−1ofU.fasciatain compared with control sediment, respectively, strongly related with Chloroflexi, Spirochaetes, Proteobacteria and Bacteroidetes hosts. In addition,U.fasciatafavored the decline of absolute gene numbers of some tetracycline resistance genes (tetPB,tetW,otrA,tetT,tetO) and class 1 integron-integrase gene.