Gold nanoparticle-based low limit of detection Love wave biosensor for carcinoembryonic antigens
Gold nanoparticle-based low limit of detection Love wave biosensor for carcinoembryonic antigens
复制标题
基于金纳米粒子的低检测限乐福波癌胚抗原生物传感器
DOI：
10.1016/j.bios.2017.04.012
复制
发表时间：
2017-09-15
影响因子：
12.6
通讯作者：
Bhethanabotla, Venkat R.
中科院分区：
文献类型：
--
作者：
Li, Shuangming;Wan, Ying;Bhethanabotla, Venkat R.
在这项工作中，描述了一个用于检测癌症相关生物标志物癌胚抗原（CEA）的Love wave生物传感平台。将ST 90°- x石英Love”波器件与一层SiO2波导与金纳米粒子（Au NPs）结合，放大声波传感器的质量负载效应，达到37 pg/mL的检测限。该策略包括用抗cea抗体偶联物修饰Au NPs，在三明治免疫分析中形成纳米探针。Love波生物传感器的检测限为9.4 ng/mL。检测极限降低了2-3个数量级，使SAW平台进入了对临床诊断有用的范围。测量电子和微流体很容易构建声波生物传感器，如这里描述的Love波设备，为癌症生物标志物的护理点应用提供了强大的平台。
In this, work, a Love wave biosensing platform is described for detecting cancer-related biomarker carcinoembryonic antigen (CEA). An ST 90 degrees-X quartz Love "wave device with a layer of SiO2 waveguide was combined with gold nanoparticles (Au NPs) to amplify the mass loading effect of the acoustic wave sensor to achieve a limit of detection of 37 pg/mL. The strategy involves modifying the Au NPs with anti-CEA antibody conjugates to form nanoprobes in a sandwich immunoassay. The unamplified detection limit of the Love wave biosensor is 9.4 ng/mL. This 2-3 order of magnitude reduction in the limit of detection brings the SAW platform into the range useful for clinical diagnosis. Measurement electronics and microfluidics are easily constructed for acoustic wave biosensors, such as the Love wave device described here, allowing for robust platforms for point of care applications for cancer biomarkers in general.