Competitive protein-binding assay for 25-hydroxycholecalciferol.
Competitive protein-binding assay for 25-hydroxycholecalciferol.
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25-羟基胆钙化醇的竞争性蛋白质结合测定。
DOI：
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
E. Kodicek
中科院分区：
文献类型：
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作者：
S. Edelstein;M. Charman;D. E. M. Lawson;E. Kodicek
1.建立了血浆25-羟胆钙醇的竞争性蛋白结合分析方法，采用Sephadex LH-20柱分离血浆25-羟胆钙醇，以大鼠血清中部分纯化的蛋白为结合蛋白。2.18名正常男性和女性血浆25-羟胆钙醇的平均值为38±SD 14pmol/ml(15-2±5-6 ng/ml)。3.4名男性志愿者在饮食中每天补充26·1nmol(10μg)胆钙醇2个月后，血浆中25-羟胆钙醇水平显著升高。4.血浆25-羟胆钙素竞争性蛋白结合法测定结果与生物测定法测定结果高度相关。
1. A competitive protein-binding assay for plasma 25-hydroxycholecalciferol was developed, using Sephadex LH-20 columns for the isolation of the sterol and a partially purified protein from rat serum as the binding protein. 2. The mean plasma 25-hydroxycholecalciferol in eighteen male and female normal volunteers was 38 ± SD 14 pmol/ml (15–2 ± 5–6 ng/ml). 3. Levels of 25-hydroxycholecalciferol in plasma of four male volunteers maintained for 2 months on a diet supplemented with 26·1 nmol (10 μg) of cholecalciferol a day were found to be significantly higher. 4. High correlation was established between plasma 25-hydroxycholecalciferol values obtained by the competitive protein-binding assay and values obtained by bioassay.
维生素 D 结合蛋白。
影响因子：
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作者：
HaddadJr,JG
通讯作者：
HaddadJr,JG