EFFECTS OF 2-DEOXYGLUCOSE, GLUCOSAMINE, AND MANNOSE ON CELL-FUSION AND GLYCOPROTEINS OF HERPES-SIMPLEX VIRUS
EFFECTS OF 2-DEOXYGLUCOSE, GLUCOSAMINE, AND MANNOSE ON CELL-FUSION AND GLYCOPROTEINS OF HERPES-SIMPLEX VIRUS
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DOI：
10.1128/jvi.18.2.644-651.1976
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发表时间：
1976-01-01
影响因子：
5.4
通讯作者：
PERSON, S
中科院分区：
文献类型：
--
作者：
KNOWLES, RW;PERSON, S
2-脱氧葡萄糖和葡糖胺被发现抑制细胞融合所造成的合胞体突变的单纯疱疹病毒，并抑制糖基化的病毒糖蛋白在感染的细胞。当感染过程中也存在甘露糖时，基本上防止了由2-脱氧葡萄糖引起的融合抑制和糖基化抑制。当糖基化被抑制时，在十二烷基硫酸钠-聚丙烯酰胺凝胶上的主要糖蛋白区域发现三条新带。这些条带可能是正常糖蛋白的前体。在2-脱氧葡萄糖、葡糖胺和甘露糖存在下融合和糖基化之间的相关性表明，如果细胞的糖蛋白具有显著降低的碳水化合物含量，则细胞不能融合。
2-Deoxyglucose and glucosamine were found to inhibit cell fusion caused by a syncytial mutant of herpes simplex virus and to inhibit the glycosylation of viral glycoproteins in the infected cells. The inhibition of fusion and the inhibition of glycosylation caused by 2-deoxyglucose were substantially prevented when mannose was also present during infection. When glycosylation was inhibited, three new bands were found in major glycoprotein region on sodium dodecyl sulfate-polyacrylamide gels. These bands may be precursors to the normal glycoproteins. The correlation between fusion and glycosylation in the presence of 2-deoxyglucose, glucosamine, and mannose suggests that the cells cannot fuse if their glycoproteins have a considerably reduced carbohydrate content.