STIMULATION OF INSULIN SECRETION BY AMINO ACIDS
STIMULATION OF INSULIN SECRETION BY AMINO ACIDS
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DOI：
10.1172/jci105456
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发表时间：
1966-01-01
影响因子：
15.9
通讯作者：
RULL, J
中科院分区：
文献类型：
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作者：
FLOYD, JC;FAJANS, SS;RULL, J
方法以19~27岁的35名健康男性和3名健康女性为研究对象。向每位参与者解释了研究的目标、测试材料的性质以及它们对测试对象的预期影响。因此，所有受试者都对进行测试给予充分知情同意。每个受试者都继续正常饮食，但在测试前至少 8 小时没有进食。使用尽可能多的测试材料对受试者进行测试，并且在可行的情况下，使用相同的氨基酸溶液进行重复测试。单独的氨基酸和2至10种氨基酸的混合物以2.5至30g的量静脉内施用。以30克量施用的10种必需氨基酸的混合物和6至8种氨基酸的混合物（溶液A至G）的组成在表I中给出。其他混合物由2、3、4、5或8种氨基酸组成，其量与30克10种氨基酸的混合物中存在的量相同。在输注某些单独的氨基酸期间或之后，一些受试者经历了不良反应反应，其详细信息在结果中给出。当反应发生时，不进行等于或超过该剂量水平的进一步测试，也不建议进行。必需的 I-氨基酸 2 以晶体形式获得，为纯氨基酸，但赖氨酸除外，赖氨酸为盐酸盐。在某些情况下，组氨酸和精氨酸也以盐酸盐形式获得。氨基酸水溶液按浓度制备
MethodsThirty-five healthy males and 3 healthy females between the ages of 19 and 27 years served as subjects for this study. The objectives of the study, the nature of the test materials, and their expected effect upon the test subject were explained to each participant. Fully informed consent for the performance of the tests was thus given by all subjects. Each subject continued his usual diet but took no food for at least 8 hours before testing. A subject was tested with as many of the test materials as possible, and, when feasible, repetitive testing with the same amino acid solutions was performed. In-dividual amino acids and mixtures of 2 to 10 amino acids were administered intravenously in amounts of from 2.5 to 30 g. The composition of the mixture of 10 essential amino acids and of mixtures of 6 to 8 amino acids (solutions A through G), administered in 30-g amounts, is given in Table I. Other mixtures were made up of 2, 3, 4, 5, or 8 amino acids in the same amounts in which they had been present in the 30 g of the mixture of 10 amino acids.During or after the infusion of some individual amino acids a few subjects experienced untoward reactions, the details of which are given in the Results. When reac-tions occurred, further testing at or beyond that dose level was not performed and is not recommended. Essential I-amino acids 2 were obtained in crystalline form as the pure amino acids except for lysine, which was the hydrochloride. In some instances histidine and arginine also were obtained as the hydrochloride. Aque-ous amino acid solutions were prepared in concentrations