Imaging nanoparticles in cells by nanomechanical holography
Imaging nanoparticles in cells by nanomechanical holography
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DOI：
10.1038/nnano.2008.162
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发表时间：
2008-08-01
影响因子：
38.3
通讯作者：
Thundat, Thomas
中科院分区：
文献类型：
--
作者：
Tetard, Laurene;Passian, Ali;Thundat, Thomas
纳米材料具有潜在的医学应用，例如在药物输送领域，其可能的副作用和细胞毒性目前正在受到关注(1,2)。纳米颗粒的吸入受到极大关注，因为纳米颗粒很容易雾化。因此，能够以纳米分辨率可视化细胞结构内局部纳米粒子群的成像技术非常重要(3)。在这里，我们表明，可以使用称为扫描近场超声全息术的扫描探针显微镜技术来探测从暴露于单壁碳纳米角的小鼠获得的细胞。在细胞内部观察到纳米角，并使用显微拉曼光谱进一步证实了这一点。扫描近场超声全息术是探测生物系统中工程纳米材料相互作用的有用技术，这将极大地有益于药物输送和纳米毒理学领域。
Nanomaterials have potential medical applications, for example in the area of drug delivery, and their possible adverse effects and cytotoxicity are curently receiving attention(1,2). Inhalation of nanoparticles is of great concern, because nanoparticles can be easily aerosolized. Imaging techniques that can visualize local populations of nanoparticles at nanometre resolution within the structures of cells are therefore important(3). Here we show that cells obtained from mice exposed to single-walled carbon nanohorns can be probed using a scanning probe microscopy technique called scanning near field ultrasonic holography. The nanohorns were observed inside the cells, and this was further confirmed using micro Raman spectroscopy. Scanning near field ultrasonic holography is a useful technique for probing the interactions of engineered nanomaterials in biological systems, which will greatly benefit areas in drug delivery and nanotoxicology.