Empirically Determined Vascular Smooth Muscle Cell Mechano-Adaptation Law.
Empirically Determined Vascular Smooth Muscle Cell Mechano-Adaptation Law.
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DOI：
10.1115/1.4036454
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发表时间：
2017-07
期刊：
影响因子：
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通讯作者：
Kerianne E. Steucke;Zaw Win;Taylor R. Stemler;Emily E. Walsh;Jennifer L. Hall;Patrick W. Alford
中科院分区：
文献类型：
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作者：
Kerianne E. Steucke;Zaw Win;Taylor R. Stemler;Emily E. Walsh;Jennifer L. Hall;Patrick W. Alford
心血管疾病可以改变血管系统的力学环境，导致力学适应性生长和重塑。动脉机械适应的预测模型可以改善心血管疾病的患者治疗和结局。血管尺度的机械适应包括细胞和细胞外基质的重塑。在这里，我们的目的是实验测量和表征的现象学机械适应法的血管平滑肌细胞（VSMCs）内的动脉。为此，我们开发了一种高度受控和可重复的系统，用于将应变的慢性阶跃变化应用于具有体内类似结构的个体VSMC，并跟踪时间细胞应力演变。我们发现，一个简单的线性增长规律是能够捕捉到动态应力演变的VSMCs在这种机械扰动。这些结果提供了一个初步的框架，发展临床相关模型的血管重塑，包括VSMC适应。
Cardiovascular disease can alter the mechanical environment of the vascular system, leading to mechano-adaptive growth and remodeling. Predictive models of arterial mechano-adaptation could improve patient treatments and outcomes in cardiovascular disease. Vessel-scale mechano-adaptation includes remodeling of both the cells and extracellular matrix. Here, we aimed to experimentally measure and characterize a phenomenological mechano-adaptation law for vascular smooth muscle cells (VSMCs) within an artery. To do this, we developed a highly controlled and reproducible system for applying a chronic step-change in strain to individual VSMCs with in vivo like architecture and tracked the temporal cellular stress evolution. We found that a simple linear growth law was able to capture the dynamic stress evolution of VSMCs in response to this mechanical perturbation. These results provide an initial framework for development of clinically relevant models of vascular remodeling that include VSMC adaptation.