Choriocapillaris photodynamic therapy using indocyanine green
Choriocapillaris photodynamic therapy using indocyanine green
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DOI：
10.1016/s0002-9394(01)01138-2
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发表时间：
2001-10-01
影响因子：
4.2
通讯作者：
Cardillo, JA
中科院分区：
文献类型：
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作者：
Costa, RA;Farah, ME;Cardillo, JA
目得：为了评价使用吲哚菁绿色的光动力疗法用于脉络膜新生血管闭塞的潜力，作者研究了光动力疗法诱导的兔脉络膜毛细血管层光血栓形成的效率和附带损害。采用眼底照相、荧光素血管造影、光镜和透射电镜观察吲哚菁绿色作为光敏剂，并评估由此产生的附带损害。传递系统由一个调整到810 nm的红外二极管激光器组成，接近吲哚菁绿色的最大吸收峰。结果：在吲哚菁绿色剂量为10和20 mg/kg，辐射低至6.3 J/cm（2）时，实现了脉络膜毛细血管闭塞。当用10 mg/kg的吲哚菁绿色剂量进行光动力疗法时，对神经视网膜的损伤是最小的。只有内部感光节段表现出变性，可能继发于脉络膜缺血。Bruch膜保持完整。与其他光敏剂一样，视网膜色素上皮总是受损。大脉络膜血管暂时闭塞发生在这两个染料doses.CONCLUSIONS：在这项实验研究中，使用吲哚菁绿色和810 nm的光照射的光动力疗法产生内皮细胞结合的腔内光血栓形成，与保存的视网膜结构和视觉细胞的损失最小。膜的靶向性、亲水性和荧光特性以及在805 nm处的活化表明吲哚菁绿色作为脉络膜新生血管的潜在光敏剂。这些综合考虑指向使用吲哚菁绿色的光动力疗法用于治疗脉络膜血管疾病的进一步研究。(C)2001年，Elsevier Science Inc. All rights reserved.
PURPOSE: To evaluate the potential of photodynamic therapy using indocyanine green for occlusion of choroidal neovascularization, the authors studied efficiency and collateral damage of photodynamic therapy-induced photothrombosis in the rabbit choriocapillary layer.METHODS: Fundus photography, fluorescein angiography, and light and transmission electron microscopy were used to study the efficiency of photodynamic therapy-induced photothrombosis using indocyanine green as the photosensitizer, and to assess the resultant collateral damage. The delivery system consisted of a modified infrared diode laser tuned to 810 nm, near the maximum absorption peak of indocyanine green.RESULTS: Choriocapillary occlusion was achieved at indocyanine green doses of 10 and 20 mg/kg and a radiant as low as 6.3 J/cm(2). When photodynamic therapy was performed with indocyanine green doses of 10 mg/kg, damage to the neural retina was minimal. Only inner photoreceptor segments showed degeneration, probably secondary to choroidal ischemia. Bruch membrane remained intact. Retinal pigment epithelium was invariably damaged, as seen with other photosensitizers. Temporary occlusion of large choroidal vessels occurred at both dye doses.CONCLUSIONS: In this experimental study, photodynamic therapy using indocyanine green and 810-nm light irradiation produced endothelium-bound intraluminal photothrombosis, with preservation of the retinal architecture and minimal loss of visual cells. Membrane targetability, hydrophilic and fluorescent properties, and activation at 805 nm suggest indocyanine green as a potential photosensitizer for choroidal neovascularization. These combined considerations point toward further study of photodynamic therapy using indocyanine green for the treatment of choroidal vascular disease. (C) 2001 by Elsevier Science Inc. All rights reserved.