Comparison of the vaccine efficacy of γ-irradiated Schistosoma japonicum cercariae with the defined antigen Sj62(IrV-5) in pigs
Comparison of the vaccine efficacy of γ-irradiated Schistosoma japonicum cercariae with the defined antigen Sj62(IrV-5) in pigs
复制标题
DOI：
10.1016/s0304-4017(01)00483-6
复制
发表时间：
2001-09-12
影响因子：
2.6
通讯作者：
Zhang, YB
中科院分区：
文献类型：
--
作者：
Bickle, QD;Bogh, HO;Zhang, YB
研制一种能保护人和家畜的人畜共患感染宿主的抗日本血吸虫疫苗，将是在常规控制方法未能阻断传播的地区控制这种感染的一种宝贵工具。猪是S.日本血吸虫与人类在解剖学和免疫学上的相似性，使其成为研究血吸虫的潜在宿主。特别是日本血吸虫和一般的拟海蛞蝓。放射减毒尾蚴在实验性巨噬细胞体病中诱导免疫是非常有效的，并且有关于重组衍生的个体抗原对巨噬细胞体的部分保护的有希望的报道。在本研究中，我们已经着手建立一个协议，诱导保护与γ尾蚴在猪和评估的保护能力的重组和裸DNA制剂的Sj 62，62 kDa的区域的S。日本肌球蛋白相应的S. mansoni版本或Sj 62，重组IrV-5，先前已涉及S.用20 krad辐照的2000条尾蚴，以2周的间隔，用作为掺入弗氏佐剂的重组体（rSj 62）、胶束制剂或作为裸DNA构建体的Sj 62，对猪组进行三次免疫。用辐照的尾蚴接种疫苗没有诱导显著的抗Sj 62抗体，但是在用2000个尾蚴肌肉内攻击后，接种疫苗的猪显示>95%的抗性，如通过减少的粪便卵输出、蠕虫组织卵负荷以及减少的间隔纤维化所评估的。用每种Sj 62制剂免疫诱导显著的抗Sj 62抗体应答，最高滴度（> 12，800）是用弗氏制剂，但没有一个Sj 62免疫组显示出对攻击的显著抗性。这些数据表明，Sj 62作为一种疫苗候选人的希望不大。(C)2001 Elsevier Science B. V.保留所有权利。
Development of a vaccine against Schistosoma japonicum which can protect both man and the domestic animal zoonotic reservoirs of infection would be an invaluable tool in attempts to control this infection in those areas in which conventional control methods have failed to break transmission. The pig is a natural host of S. japonicum and because of its anatomical and immunological similarities to humans, it is a potentially valuable host for studies on S. japonicum in particular and schistosomes in general. Radiation-attenuated cercariae are highly effective in inducing immunity in experimental schistosomosis and there are promising reports of partial protection against schistosomes with recombinant-derived individual antigens. In the present stud, we have set out to establish a protocol for inducing protection with gamma cercariae in pigs and to assess the protective capacity of recombinant and naked DNA formulations of Sj62, a 62 kDa region of S. Japonicum myosin. The corresponding S. mansoni version or Sj62, recombinant IrV-5, has previously been implicated in irradiated vaccine immunity in S. mansoni infections and has been shown to induce high levels of immunity in a variety of hosts.Groups of pigs were immunised three times at 2-week intervals with 2000 cercariae irradiated at 20 krad, with Sj62 as a recombinant (rSj62) incorporated in Freund's adjuvant, a micellar preparation, or as a naked DNA construct. Vaccination with irradiated cercariae did not induce significant anti-Sj62 antibody but following intramuscular challenge with 2000 cercariae, the vaccinated pigs showed >95% resistance as assessed by reduced faecal egg output, worm tissue egg burdens and also reduced septal fibrosis. Immunisation with each of the Sj62 formulations induced significant anti-Sj62 antibody responses, the highest titre (>12,800) being with the Freund's preparation but none of the Sj62-immunised groups showed significant resistance to challenge. The data suggest that Sj62 shows little promise as a vaccine candidate for schistosomosis. (C) 2001 Elsevier Science B.V. All rights reserved.