ACTIVATION OF HUMAN FIBROBLASTS BY CHRONIC RADIATION RATHER THAN ACUTE RADIATION
ACTIVATION OF HUMAN FIBROBLASTS BY CHRONIC RADIATION RATHER THAN ACUTE RADIATION
复制标题
通过慢性辐射而不是急性辐射激活人体成纤维细胞
DOI：
10.1093/rpd/ncac065
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发表时间：
2022
影响因子：
1
通讯作者：
Ushiyama A
中科院分区：
文献类型：
--
作者：
Shimura T;Zaharieva E;Sasatani M;Kawai H;Kamiya K;Ushiyama A
癌相关成纤维细胞（CAF）是一种活化型成纤维细胞，是肿瘤微环境（TME）中促进肿瘤发生和发展的主要基质细胞。我们之前报道了分次辐射而不是急性辐射会导致线粒体的进行性损伤，并增加活性氧的产生，在正常组织损伤的成纤维细胞活化中起重要作用。被激活的成纤维细胞通过与肿瘤细胞相互作用而成为CAF，促进肿瘤在体内的生长。我们在此研究了慢性辐射对成纤维细胞激活的影响。急性辐射（<2.5 Gy）不会增加健康人细胞中CAF标志物α -平滑肌肌动蛋白的表达，而慢性辐射（2.5 Gy）则会增加。结果表明，急性辐射、分次辐射和慢性辐射对成纤维细胞活化的诱导作用随辐照技术的不同而不同。这项研究强调，辐射激活成纤维细胞，通过TME的形成在辐射相关肿瘤的发展中发挥作用。
Cancer-associated fibroblast (CAF), an activated type of fibroblast, is a major stromal cell that contributes to tumor initiation and development in the tumor microenvironment (TME). We previously reported that fractionated radiation rather than acute radiation causes progressive damage to mitochondria and increases the generation of reactive oxygen species, playing an important role in the fibroblast activation in normal tissue injury. Activated fibroblasts then become CAF by interacting with tumor cells, promoting tumor growth in vivo. We here examined the chronic radiation effect on fibroblast activation. Acute radiation (<2.5 Gy) did not increase alpha-Smooth muscle actin, a CAF marker expression in healthy human cells, whereas chronic radiation (2.5 Gy) did. It can be concluded that the induction of fibroblast activation changes across acute radiation, fractionated radiation, and chronic radiation depending on the irradiation technique. This study highlights that radiation activates fibroblasts, playing a role in radiation-related tumor development via TME formation.