A fitness cost for Turnip mosaic virus to overcome host resistance
A fitness cost for Turnip mosaic virus to overcome host resistance
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DOI：
10.1016/s0168-1702(02)00031-x
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发表时间：
2002-06-01
期刊：
影响因子：
5
通讯作者：
Walsh, JA
中科院分区：
文献类型：
--
作者：
Jenner, CE;Wang, XW;Walsh, JA
将芜菁花叶病毒 (TuMV) 分离株 UK 1 的相对适合度与其他两种野生型分离株 CZE 1 和 CDN 1 的相对适合度进行了比较。分离株 CZE 1 和 CDN 1 能够克服抗性基因 TuRB01 和至少三个其他对 UK 1 有效的抗性来源的影响。还对 UK I 和具有单核苷酸变化的重组病毒的适合度进行了比较 (v35Tunos + 5570 A > G) 赋予克服 TuRB01 抗性的能力。进行了联合接种实验，在不具有已知抗性基因的植物系中，将成对分离株连续传代超过 5 个月，以检查分离株的相对适应性。在每种情况下，UK 1 在易感宿主背景中占主导地位。它在四次传代内击败了 CZE 1 和 v35Tunos + 5570 A > G，并在一次传代后击败了 CDN 1。 UK 1 更大的适应性表明，TuMV 克服芸苔属作物的抗性基因可能存在适应性成本，这可能对自然发生的分离株的频率有一定的启发，因为致病型 1 分离株比其他致病型分离株的发现频率要高得多。讨论了 TuRB01 部署的影响。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The relative fitness of the Turnip mosaic virus (TuMV) isolate UK 1 was compared with that of two other wildtype isolates CZE 1 and CDN 1. The isolates CZE 1 and CDN 1 are able to overcome the effect of the resistance gene TuRB01 and at least three other resistance sources that are effective against UK 1. Comparisons were also made between the fitness of UK I and a recombinant virus with a single nucleotide change (v35Tunos + 5570 A > G) conferring the ability to overcome TuRB01 resistance. Co-inoculation experiments were carried out where pairs of isolates were serially passaged over 5 months in a plant line possessing no known resistance genes in order to examine the relative fitness of the isolates. In each case, UK 1 dominated the mixture in the susceptible host background. It out-competed CZE 1 and v35Tunos + 5570 A > G within four passages, and CDN 1 after one passage. The greater fitness of UK 1 suggests that there may be a fitness cost to TuMV overcoming resistance genes of brassica crops, This may shed some light on the frequency of naturally occurring isolates, in that pathotype 1 isolates are found much more frequently than isolates of other pathotypes. Implications for the deployment of TuRB01 are discussed. (C) 2002 Elsevier Science B.V. All rights reserved.