Comparative proteomics of glycosomes from bloodstream form and procyclic culture form Trypanosoma brucei brucei
Comparative proteomics of glycosomes from bloodstream form and procyclic culture form Trypanosoma brucei brucei
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DOI：
10.1002/pmic.200500668
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发表时间：
2006-06-01
期刊：
影响因子：
3.4
通讯作者：
Voncken, Frank
中科院分区：
文献类型：
--
作者：
Colasante, Claudia;Ellis, Margrit;Voncken, Frank
过氧化物酶体存在于几乎每一个真核细胞中，并划分了广泛的重要代谢过程。动体寄生虫的糖体是过氧化物酶体样细胞器，其特征是存在糖酵解途径。布氏锥虫布氏锥虫的两个复制阶段，即哺乳动物血流形式（BSF）和昆虫（顺环）形式（PCF），在两种不同宿主之间切换时，在代谢方面经历了相当大的适应。这些适应也涉及糖体蛋白质组的实质性变化。通过纳米LC-ESI-Q-TOF-MS对BSF和PCF糖体进行比较（非定量）分析，验证了糖体的已知功能方面，并鉴定了新的糖体成分。
Peroxisomes are present in nearly every eukaryotic cell and compartmentalize a wide range of important metabolic processes. Glycosomes of Kinetoplastid parasites are peroxisome-like organelles, characterized by the presence of the glycolytic pathway. The two replicating stages of Trypanosoma brucei brucei, the mammalian bloodstream form (BSF) and the insect (procyclic) form (PCF), undergo considerable adaptations in metabolism when switching between the two different hosts. These adaptations involve also substantial changes in the proteome of the glycosome. Comparative (non-quantitative) analysis of BSF and PCF glycosomes by nano LC-ESI-Q-TOF-MS resulted in the validation of known functional aspects of glycosomes and the identification of novel glycosomal constituents.