DYE TRANSFER BETWEEN CELLS OF THE EMBRYONIC CHICK LENS BECOMES LESS SENSITIVE TO CO2 TREATMENT WITH DEVELOPMENT
DYE TRANSFER BETWEEN CELLS OF THE EMBRYONIC CHICK LENS BECOMES LESS SENSITIVE TO CO2 TREATMENT WITH DEVELOPMENT
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DOI：
10.1083/jcb.92.3.694
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发表时间：
1982-01-01
影响因子：
7.8
通讯作者：
GOODENOUGH, DA
中科院分区：
文献类型：
--
作者：
SCHUETZE, SM;GOODENOUGH, DA
在小鸡的3小时发育阶段14，离子电渗荧光染料的细胞间转移变得不太敏感的细胞内pH值的降低，无论是CO2或乙酸根离子。直到发育阶段14，透镜细胞之间的染料转移通过暴露于50%CO2而可逆地阻断。超过阶段14，这些细胞之间的染料转移不再可逆地被升高的P[分压]CO2阻断。电紧张耦合存在于整个透镜发育过程中，并且在任何阶段都不会被高PCO2可逆性阻断。连接透镜细胞的差距连接在发育阶段14显示形态学变化。直到第14阶段，在鸡透镜细胞之间观察到的所有间隙连接具有在常规冷冻断裂显微镜下出现浓缩或结晶的连接子组装。在第14阶段以后，小鸡透镜细胞表达与骨的间隙连接，即成年透镜间隙连接结构特征的聚集组装和分散组装。
During the 3 h developmental stage 14 in the chick, intercellular transfer of iontophoresed fluorescent dyes become less sensitive to the lowering of intracellular pH by either CO2 or acetate ions. Up to developmental stage 14, dye transfer between lens cells is reversibly blocked by exposure to 50% CO2. Beyond stage 14, dye transfer between these cells is not longer reversibly blocked by elevated P[partial pressure]CO2. Electrotonic coupling is present throughout lens development and is not reversibly blocked by high PCO2 at any stage. The gap junctions joining the lens cells show morphological changes at developmental stage 14. Up to stage 14, all gap junctions observed between chick lens cells have connexon assembies that appear condensed or crystaline following routine freeze-fracture microscopy. Beyond stage 14, chick lens cells express gap junctions with bone the condensed assemblies and the dispersed assemblies characteristic of adult lens gap-junction structre.