NG-nitro-L-arginine inhibits non-adrenergic, non-cholinergic relaxation in rabbit urethral smooth muscle.
NG-nitro-L-arginine inhibits non-adrenergic, non-cholinergic relaxation in rabbit urethral smooth muscle.
复制标题
NG-硝基-L-精氨酸抑制兔尿道平滑肌的非肾上腺素能、非胆碱能松弛。
DOI：
10.1016/0024-3205(91)90377-n
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发表时间：
1991
期刊：
影响因子：
6.1
通讯作者：
Weiss,RM
中科院分区：
文献类型：
--
作者：
Dokita,S;Morgan,WR;Wheeler,MA;Yoshida,M;Latifpour,J;Weiss,RM
电场刺激引起苯乙醯胺预收缩的雌兔尿道平滑肌条的舒张反应。河豚毒素可抑制松弛反应，但阿托品、普萘洛尔或六烃季铵不能抑制松弛反应。因此，松弛反应是由抑制性非肾上腺素能、非胆碱能神经的刺激引起的。一氧化氮生物合成抑制剂NG-硝基-L-精氨酸可抑制电诱发的舒张反应。通过添加一氧化氮的前体L-精氨酸克服了这种抑制作用。可溶性鸟苷酸环化酶抑制剂亚甲基蓝可降低松弛反应，选择性环GMP磷酸二酯酶抑制剂M & B 22948可增强松弛反应。这些数据表明，影响一氧化氮生物合成的药物与电场刺激引起的尿道松弛反应有关，并且环GMP可能介导松弛反应。
Electrical field stimulation induced a relaxation response in female rabbit urethral smooth muscle strips precontracted with phenylephrine. The relaxation response was inhibited by tetrodotoxin, but not by atropine, propranolol, or hexamethonium. The relaxation response thus results from stimulation of inhibitory non-adrenergic, non-cholinergic nerves. The electrically induced relaxation response was inhibited by an inhibitor of nitric oxide biosynthesis, NG-nitro-L-arginine. This inhibition was overcome by addition of a precursor of nitric oxide, L-arginine. An inhibitor of soluble guanylate cyclase, methylene blue, reduced the relaxation response, and a selective cyclic GMP phosphodiesterase inhibitor, M & B 22948, potentiated the relaxation response. These data indicate that agents which affect the biosynthesis of nitric oxide are associated with the urethral relaxation response evoked by electrical field stimulation, and that cyclic GMP may mediate the relaxation response.