Active DNA demethylation regulates tracheary element differentiation in Arabidopsis
Active DNA demethylation regulates tracheary element differentiation in Arabidopsis
复制标题
DNA主动去甲基化调控拟南芥导管分子分化
DOI：
10.1126/sciadv.aaz2963
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发表时间：
2020-06-01
期刊：
影响因子：
13.6
通讯作者：
Qian, Weiqiang
中科院分区：
文献类型：
--
作者：
Lin, Wei;Sun, Linhua;Qian, Weiqiang
DNA去甲基化对于DNA甲基化的消除是重要的。DNA去甲基化在植物发育中的作用仍然知之甚少。在这里，我们发现广泛的DNA去甲基化的CHH上下文周围的pericentromeric区域和DNA去甲基化的CG，CHG和CHH上下文在离散的基因组区域在异位木质部导管元件（TE）分化。尽管着丝粒周围甲基化的丧失是被动发生的，但一部分区域的DNA去甲基化依赖于由DNA糖基化酶ROS1、DML2和DML3启动的主动DNA去甲基化。ros1和rdd突变损害拟南芥幼苗幼根的异位TE分化和木质部发育。活性DNA去甲基化靶向并调节TE分化的许多基因。推测其木质部发育缺陷是由多个基因调控失调所致。我们的研究确定了主动DNA去甲基化在血管发育中的作用，并揭示了TE分化的表观遗传机制。
DNA demethylation is important for the erasure of DNA methylation. The role of DNA demethylation in plant development remains poorly understood. Here, we found extensive DNA demethylation in the CHH context around pericentromeric regions and DNA demethylation in the CG, CHG, and CHH contexts at discrete genomic regions during ectopic xylem tracheary element (TE) differentiation. While loss of pericentromeric methylation occurs passively, DNA demethylation at a subset of regions relies on active DNA demethylation initiated by DNA glycosylases ROS1, DML2, and DML3. The ros1 and rdd mutations impair ectopic TE differentiation and xylem development in the young roots of Arabidopsis seedlings. Active DNA demethylation targets and regulates many genes for TE differentiation. The defect of xylem development in rdd is proposed to be caused by dysregulation of multiple genes. Our study identifies a role of active DNA demethylation in vascular development and reveals an epigenetic mechanism for TE differentiation.