The potential of biophotonic techniques in stem cell tracking and monitoring of tissue regeneration applied to cardiac stem cell therapy
The potential of biophotonic techniques in stem cell tracking and monitoring of tissue regeneration applied to cardiac stem cell therapy
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DOI：
10.1002/jbio.200910079
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发表时间：
2009-11-01
影响因子：
2.8
通讯作者：
Matthews, Dennis L.
中科院分区：
文献类型：
--
作者：
Wilson, Brian C.;Vitkin, I. Alex;Matthews, Dennis L.
使用注射干细胞，例如，在冠状动脉闭塞后，导致缺血心脏组织的再生，已经成为治疗心脏病发作患者的一种主要的新选择。虽然一些临床试验令人鼓舞，但也有失败的情况，人们对导致结果的多种因素知之甚少。在这篇综述文章中，以缺血性心脏病的干细胞治疗的挑战为例，讨论了将生物光子学技术应用于再生医学的机遇和挑战。重点是光学成像，以跟踪干细胞的分布和命运，以及光学光谱和/或成像，以监测组织的结构重塑和由此产生的功能变化。还强调了将其中一些技术从临床前研究模式最终转移到临床所涉及的科学、技术和后勤问题。[图表].Wiley-VCH Verlag GmbH&Co.KGaA提供的干细胞治疗后缺血大鼠心肌中二次谐波产生(绿色)和双光子激发荧光(红色)的合成图像(C)2009。温海姆
The use of injected stem cells, leading to regeneration of ischemic heart tissue, for example, following coronary artery occlusion, has emerged as a major new option for managing 'heart attack' patients. While some clinical trials have been encouraging, there have also been failures and there is little understanding of the multiplicity of factors that lead to the outcome. In this overview paper, the opportunities and challenges in applying biophotonic techniques to regenerative medicine, exemplified by the challenge of stem cell therapy of ischemic heart disease, are considered. The focus is on optical imaging to track stem cell distribution and fate, and optical spectroscopies and/or imaging to monitor the structural remodeling of the tissue and the resulting functional changes. The scientific, technological, and logistics issues involved in moving some of these techniques from pre-clinical research mode ultimately into the clinic are also highlighted.[GRAPHICS].Composite image of second harmonic generation (green) and 2-photon excitation fluorescence (red) in ischemic rat myocardium following stem cell treatment (C) 2009 by WILEY-VCH Verlag GmbH & Co. KGaA. Weinheim