THE C-ELEGANS HETEROCHRONIC GENE LIN-4 ENCODES SMALL RNAS WITH ANTISENSE COMPLEMENTARITY TO LIN-14
THE C-ELEGANS HETEROCHRONIC GENE LIN-4 ENCODES SMALL RNAS WITH ANTISENSE COMPLEMENTARITY TO LIN-14
复制标题
DOI：
10.1016/0092-8674(93)90529-y
复制
发表时间：
1993-12-03
期刊：
影响因子：
64.5
通讯作者：
AMBROS, V
中科院分区：
文献类型：
--
作者：
LEE, RC;FEINBAUM, RL;AMBROS, V
lin-4对C.优美的lin-4通过负调节LIN-14蛋白的水平起作用，在第一幼虫阶段（L1）开始产生LIN-14蛋白的暂时降低。我们克隆了C。elegans lin-4基因座进行染色体步移和转化拯救。我们用了C。elegans克隆以从其他三个小杆线虫物种中分离该基因;所有四个小杆线虫克隆在功能上拯救C.优雅的比较这四个物种的lin-4基因组序列和潜在开放阅读框的定点突变表明lin-4不编码蛋白质。在C.发现lin-14 mRNA的3'非翻译区（UTR）含有与重复序列元件互补的序列，表明lin-4通过反义RNA-RNA相互作用调节lin-14的翻译。
lin-4 is essential for the normal temporal control of diverse postembryonic developmental events in C. elegans. lin-4 acts by negatively regulating the level of LIN-14 protein, creating a temporal decrease in LIN-14 protein starting in the first larval stage (L1). We have cloned the C. elegans lin-4 locus by chromosomal walking and transformation rescue. We used the C. elegans clone to isolate the gene from three other Caenorhabditis species; all four Caenorhabditis clones functionally rescue the lin-4 null allele of C. elegans. Comparison of the lin-4 genomic sequence from these four species and site-directed mutagenesis of potential open reading frames indicated that lin-4 does not encode a protein. Two small lin-4 transcripts of approximately 22 and 61 nt were identified in C. elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin-4 regulates lin-14 translation via an antisense RNA-RNA interaction.