Establishment of embryonic stem cell lines from cynomolgus monkey blastocysts produced by IVF or ICSI
Establishment of embryonic stem cell lines from cynomolgus monkey blastocysts produced by IVF or ICSI
复制标题
DOI：
10.1002/dvdy.1191
复制
发表时间：
2001-10-01
影响因子：
2.5
通讯作者：
Nakatsuji, N
中科院分区：
文献类型：
--
作者：
Suemori, H;Tada, T;Nakatsuji, N
人类胚胎干细胞被认为是一种有价值的来源，可以通过控制胚胎干细胞的生长和分化来产生治疗疾病的ES来源的治疗备用组织。从非人灵长类动物来源的ES细胞可能是了解其在体内和体外分化的调控机制的有力工具。从食蟹猴体外受精(IVF)和卵胞浆内单精子注射(ICSI)获得的囊胚中建立了4个ES细胞系。ES细胞的特征是表达碱性磷酸酶和阶段特异性胚胎抗原-4等特异性标志物。即使经过6个多月的培养，它们仍能成功地保持未分化状态，并具有正常的核型。皮下注射SCID小鼠后，形成了含有外胚层、中胚层和内胚层衍生物的畸胎瘤，证实了多潜能的存在。通过使用组织特异性抗体的免疫组织化学分析，鉴定分化为各种组织。因此，我们建立了广泛应用于实验动物的猴源多能ES细胞系。这些品系可能是临床前干细胞研究的有用资源，包括将同种异体移植到疾病的猴子模型中。(C)2001年Wiley-Liss，Inc.
Human embryonic stem (ES) cells are predicted to be a valuable source for producing ES-derived therapeutic spare tissues to treat diseases by controlling their growth and differentiation. To understand the regulative mechanisms of their differentiation in vivo and in vitro, ES cells derived from nonhuman primates could be a powerful tool. We established four ES cell lines from cynomolgus monkey (Macaca fascicularis) blastocysts produced by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). The ES cells were characterized by the expression of specific markers such as alkaline phosphatase and stage-specific embryonic antigen-4. They were successfully maintained in an undifferentiated state and with a normal karyotype even after more than 6 months of culture. Pluripotential competence was confirmed by the formation of teratomas containing ectoderm-, mesoderm-, and endoderm-derivatives after subcutaneous injection into SCID mice. Differentiation to a variety of tissues was identified by immunohistochemical analyses using tissue-specific antibodies. Therefore, we established pluripotent ES cell lines derived from monkeys that are widely used as experimental animals. These lines could be a useful resource for preclinical stem cell research, including allogenic transplantation into monkey models of disease. (C) 2001 Wiley-Liss, Inc.