Preweaning enrichment has no lasting effects on adult hippocampal neurogenesis in four-month-old mice
Preweaning enrichment has no lasting effects on adult hippocampal neurogenesis in four-month-old mice
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DOI：
10.1046/j.1601-1848.2001.00009.x
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发表时间：
2002-01-01
影响因子：
2.5
通讯作者：
Kempermann, G
中科院分区：
文献类型：
--
作者：
Kohl, Z;Kuhn, HG;Kempermann, G
由于生活在一个丰富的环境和体力活动刺激成年小鼠海马神经发生，我们endeetly检查是否断奶前的丰富，感官丰富的范式与非常有限的体力活动，有类似的影响神经发生以后的生活。从出生后第7天到第21天，将小鼠从母体中取出，并暴露于各种感官刺激。在4月龄时，当监测自发活动时，可以在先前富集和未富集的动物之间发现显著差异。富集的小鼠移动更长的距离，并花更多的时间在一个明确的中心区域的开放领域。用溴脱氧尿苷（BrdU）标记齿状回增殖细胞，观察成年神经发生。两组的细胞增殖、新生细胞存活和净神经发生相似。总颗粒细胞计数的体积测量和体视学评估显示两组之间齿状回的大小没有差异。因此，与断奶后富集相反，断奶前富集对成年神经发生没有持久的可测量的影响。造成这种影响的参数之一可能是断奶前富集中缺乏体力活动。由于体力活动是断奶后营养的一个组成部分，它可能是引起对环境刺激的神经性反应的必要因素。该结果也可能意味着基线成年海马神经发生是独立的断奶前富集诱导的变化，可能不会有助于丰富的动物中看到的持续类型的可塑性。
Since both living in an enriched environment and physical activity stimulate hippocampal neurogenesis in adult mice, we endeavored to examine whether pre-weaning enrichment, a sensory enrichment paradigm with very limited physical activity, had similar effects on neurogenesis later in life. Mice were removed from the dams for periods of increasing length from postnatal day 7 to 21, and exposed to a variety of sensory stimuli. At the age of 4 months, significant differences could be found between previously enriched and non-enriched animals when spontaneous activity was monitored. Enriched mice moved longer distances, and spent more time in a defined center zone of the open field. Adult neurogenesis was examined by labeling proliferating cells in the dentate gyrus with bromodeoxyuridine (BrdU). Cell proliferation, survival of the newborn cells, and net neurogenesis were similar in both groups. Volumetric measurements and stereological assessment of total granule cell counts revealed no difference in size of the dentate gyrus between both groups. Thus, in contrast to postweaning enrichment, preweaning enrichment had no lasting measurable effect on adult neurogenesis. One of the parameters responsible for this effect might be the lack of physical activity in preweaning enrichment. As physical activity is an integral part of postweaning enrichment, it might be a necessary factor to elicit a neurogenic response to environmental stimuli. The result could also imply that baseline adult hippocampal neurogenesis is independent of the changes induced by preweaning enrichment and might not contribute to the sustained types of plasticity seen in enriched animals.