Molecular weight determinations by gel‐permeation chromatography and viscometry
Molecular weight determinations by gel‐permeation chromatography and viscometry
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通过凝胶渗透色谱法和粘度测定法测定分子量
DOI：
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发表时间：
1973
期刊：
影响因子：
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通讯作者：
B. Coulter
中科院分区：
文献类型：
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作者：
A. L. Spatorico;B. Coulter
水动力体积概念可以有效地用于凝胶渗透色谱（GPC）和粘度数据来估计各种聚合物的分子量。一致性在绝对值的±5-10%以内，因此在许多用途上是令人满意的。研究发现，在估计所研究的五种情况的分子量方面，迭代计算机技术和Funt和Hornof开发的用于分析gpc -粘度数据的方法是等效的。后者很容易使用，但仅限于感兴趣的样本和GPC校准标准具有近似相等的Mark-Houwink参数的情况。由于GPC测量通常在热力学良好的溶剂中进行，因此该方法的一般适用性不会受到损害。利用聚合物链的未扰动尺寸来估计各种聚合物的分子量并不像上述技术那样令人满意。这种方法通常给出有偏差的分子量值（一贯低或一贯高）。与绝对值的吻合度从10%到30%不等。因此，我们认为，基于水动力体积概念的任何一种技术都可以比基于无扰动尺寸的处理更有效地用于估计一系列聚合物的分子量。
The hydrodynamic volume concept can be used effectively with gel-permeation chromatographic (GPC) and viscosity data to estimate the molecular weight of a variety of polymers. Agreement is within ±5–10% of the absolute values and thus is satisfactory for many purposes. An iterative computer technique and a method developed by Funt and Hornof for analyzing GPC–viscosity data were found to be equivalent with respect to estimating the molecular weights for the five cases studied. The latter is easily employed but restricted to the case where the sample of interest and the GPC calibration standards have approximately equal Mark-Houwink parameters. Since GPC measurements are commonly performed in thermodynamically good solvents, the general applicability of the method is not impaired. Using the unperturbed dimensions of the polymer chain to estimate the molecular weight of a variety of polymers was not as satisfactory as the above techniques. This approach generally gave biased molecular weight values (consistently low or consistently high). Agreement with the absolute values ranged from 10 to 30%. We therefore believe that either of the techniques based on the hydrodynamic volume concept can be used more effectively to estimate the molecular weight of a series of polymers than the treatment based on the unperturbed dimension.