In vitro imaging of embryonic stem cells using multiphoton luminescence of gold nanoparticles
In vitro imaging of embryonic stem cells using multiphoton luminescence of gold nanoparticles
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发表时间：
2007-12
影响因子：
8
通讯作者：
D. Nagesha;G. Laevsky;P. Lampton;R. Banyal;C. Warner;C. DiMarzio;S. Sridhar
中科院分区：
文献类型：
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作者：
D. Nagesha;G. Laevsky;P. Lampton;R. Banyal;C. Warner;C. DiMarzio;S. Sridhar
非线性光学技术和材料（如量子阱、纳米线和贵金属纳米颗粒）的最新进展导致了细胞成像的进步，其中各种纳米颗粒已被证明可以改善体外和体内的可视化。在这篇论文中，我们展示了利用多光子光致发光技术对两种不同细胞类型——盘状盘骨和小鼠胚胎干细胞——的金纳米颗粒进行体外成像。通过观察纳米颗粒，我们发现胚胎干细胞在金纳米颗粒存在的情况下能够维持其增殖能力。利用金纳米粒子进行多光子发光的优势对干细胞增殖实验和体外分化监测实验具有重要意义。
Recent advances in nonlinear optical techniques and materials such as quantum wells, nanowires and noble-metal nanoparticles have led to advances in cellular imaging wherein various nanoparticles have been shown to improve both in vitro and in vivo visualization. In this paper, we demonstrate in vitro imaging using multi-photon photoluminescence of gold nanoparticles from two different cell types – Dictyostelium discoideum and mouse embryonic stem cells. By observing nanoparticles we show that embryonic stem cells maintained their ability to proliferate for several passages while grown in the presence of gold nanoparticles. The advantages of multi-photon luminescence using gold nanoparticles have important implications for use in stem cell proliferation experiments and in vitro experiments to monitor differentiation.