Analysis of polysomal mRNA populations of mouse oocytes and zygotes: dynamic changes in maternal mRNA utilization and function.
Analysis of polysomal mRNA populations of mouse oocytes and zygotes: dynamic changes in maternal mRNA utilization and function.
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DOI：
10.1016/j.ydbio.2006.06.024
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发表时间：
2006-10
影响因子：
2.7
通讯作者：
S. Potireddy;R. Vassena;B. Patel;K. Latham
中科院分区：
文献类型：
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作者：
S. Potireddy;R. Vassena;B. Patel;K. Latham
哺乳动物胚胎的转录激活是以循序渐进的方式进行的。在小鼠中，它开始于单细胞晚期，随后是早期两细胞阶段的小波激活，然后是晚期两细胞阶段的主要基因组激活事件(MGA)。细胞动态平衡、新陈代谢、细胞周期和发育事件在MGA之前是通过翻译的母体转录序列中依赖时间的变化来编排的。许多优雅的研究已经证明了母体信使核糖核酸(MmRNA)的重要性以及它对发育的正确招募。许多其他研究已经阐明了一些调节MmRNA利用的分子机制。然而，无论是被招募的完整的mRNAs序列，还是导致暂时不同的招募模式的调控机制，都没有得到很好的表征。我们提出了一个全面的分析，在从卵母细胞到晚期单细胞期胚胎的过渡过程中，合子多倍体mRNA群体的母体成分发生了变化。我们观察到翻译后的MmRNAs功能类的全局转变和潜在顺式调控机制的明显变化。
Transcriptional activation in mammalian embryos occurs in a stepwise manner. In mice, it begins at the late one-cell stage, followed by a minor wave of activation at the early two-cell stage, and then the major genome activation event (MGA) at the late two-cell stage. Cellular homeostasis, metabolism, cell cycle, and developmental events are orchestrated before MGA by time-dependent changes in the array of maternal transcripts being translated. Many elegant studies have documented the importance of maternal mRNA (MmRNA) and its correct recruitment for development. Many other studies have illuminated some of the molecular mechanisms regulating MmRNA utilization. However, neither the complete array of recruited mRNAs nor the regulatory mechanisms responsible for temporally different patterns of recruitment have been well characterized. We present a comprehensive analysis of changes in the maternal component of the zygotic polysomal mRNA population during the transition from oocyte to late one-cell stage embryo. We observe global transitions in the functional classes of translated MmRNAs and apparent changes in the underlying cis-regulatory mechanisms.