Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria.
Nitrous oxide production in soil isolates of nitrate-ammonifying bacteria.
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DOI：
10.1111/j.1758-2229.2011.00302.x
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发表时间：
2012-02
影响因子：
3.3
通讯作者：
M. Stremińska;H. Felgate;G. Rowley;D. Richardson;E. Baggs
中科院分区：
文献类型：
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作者：
M. Stremińska;H. Felgate;G. Rowley;D. Richardson;E. Baggs
在这里，我们提供了第一个示范的潜力N2 O生产土壤中分离的硝酸盐氨化细菌在不同的C和N的可用性，从模型菌株的特征。在不同的C-与-NO3（-）比率、NO3（-）限制（5 mM）和NO3（-）充足（22 mM）条件下，在分批和连续（恒化器）培养中检测土壤分离的芽孢杆菌属和柠檬酸杆菌属还原NO3（-）和产生NH 4（+）、NO2（-）和N2 O的潜力。C与NO3（-）的比例对硝酸盐氨化的产物有重要影响，在分批培养中，在低C与NO3（-）的比例下，NO2（-）而不是NH 4（+）是主要产物。在恒化器培养物中，在C限制/NO3充足条件下，N2 O产生最大，并伴随着高NO2（-）产生。在C与NO3 -N比例较低（5比1和10比1）的培养基中，芽孢杆菌和柠檬酸杆菌可将高达2.7%或5.0%的NO3（-）还原为N2 O，但在较高的C与NO3（-）比（25比1和50比1）下，这些还原效率仅为0.1%或0.7%。由于最高的N2 O生产没有发生在相同的C-到-NO3（-）的条件下，最高的NH 4（+）的生产，我们建议重新评估可能是必要的环境条件下，硝酸盐氨化有助于N2 O从土壤中排放。
Here we provide the first demonstration of the potential for N2 O production by soil-isolated nitrate-ammonifying bacteria under different C and N availabilities, building on characterizations informed from model strains. The potential for soil-isolated Bacillus sp. and Citrobacter sp. to reduce NO3 (-) , and produce NH4 (+) , NO2 (-) and N2 O was examined in batch and continuous (chemostat) cultures under different C-to-NO3 (-) ratios, NO3 (-) -limiting (5 mM) and NO3 (-) -sufficient (22 mM) conditions. C-to-NO3 (-) ratio had a major influence on the products of nitrate ammonification, with NO2 (-) , rather than NH4 (+) , being the major product at low C-to-NO3 (-) ratios in batch cultures. N2 O production was maximum and accompanied by high NO2 (-) production under C-limitation/NO3 -sufficiency conditions in chemostat cultures. In media with lower C-to-NO3 -N ratios (5- and 10-to-1) up to 2.7% or 5.0% of NO3 (-) was reduced to N2 O by Bacillus sp. and Citrobacter sp., respectively, but these reduction efficiencies were only 0.1% or 0.7% at higher C-to-NO3 (-) ratios (25- and 50-to-1). As the highest N2 O production did not occur under the same C-to-NO3 (-) conditions as highest NH4 (+) production we suggest that a re-evaluation may be necessary of the environmental conditions under which nitrate ammonification contributes to N2 O emission from soil.